Altered antioxidant status in peripheral skeletal muscle of patients with COPD

H.R. Gosker, A. Bast, G.R.M.M. Haenen, M.A.J.G. Fischer, G.J. van der Vusse, E.F.M. Wouters, A.M.W.J. Schols

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Abstract

Despite the growing field of interest in the role of pulmonary oxidative stress in chronic obstructive pulmonary disease (COPD), barely any data are available with respect to antioxidant capacity in the peripheral musculature of these patients. The main objective of this study was to assess in detail the antioxidant status in skeletal muscle of patients with COPD. Biopsies from the vastus lateralis of 21 patients with COPD and 12 healthy age-matched controls were analysed. Total antioxidant capacity, vitamin E, glutathione, and uric acid levels were determined and the enzyme activities of superoxide dismutase, glutathione reductase, glutathione peroxidase, and glutathione-S-transferase were measured. Malondialdehyde was measured as an index of lipid peroxidation. The total antioxidant capacity and the uric acid levels were markedly higher in COPD patients than in healthy controls (25%, P = 0.006 and 24%, P = 0.029, respectively). Glutathione-S-transferase activity was also increased (35%; P = 0.044) in patients compared to healthy subjects. Vitamin E level was lower in patients than in controls (P
Original languageEnglish
Pages (from-to)118-125
JournalRespiratory Medicine
Volume99
DOIs
Publication statusPublished - 1 Jan 2005

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