Alterations in the structural characteristics of rectus abdominis muscles caused by diabetes and pregnancy: A comparative study of the rat model and women

Giovana Vesentini; Angélica M.P. Barbosa; Débora C. Damasceno; Gabriela Marini; Fernanda Piculo; Selma M.M. Matheus; Raghavendra L.S. Hallur; Sthefanie K. Nunes; Bruna B. Catinelli; Claudia G. Magalhães; Roberto Costa; Joelcio F. Abbade; José E. Corrente; Iracema M.P. Calderon; Marilza V.C. Rudge; F. P. Souza; A. Hijas; L. Sobrevia; M. S. Palma; C. F.O. Graeff; R. K. Arni; R. D. Herculano; D. F. Salvadori; M. Dal Pai Silva; S. A.M. Lima; C. Emanueli; S. Felisbino; W. Barbosa; A. Atallah; M. J.B. Girão; Z. Di Bella; S. M. Uchoa; S. Payão; B. Berghman; R. De Bie; B. Junginger; F. C.B. Alves; P. S. Rossignoli; C. B. Prudencio; M. I.G. Orlandi; M. I. Gonçalves; B. V. Sarmento; F. A. Pinheiro; C. I. Sartorão; S. B.C.V. Quiroz; D. R.A. Reyes; E. M.A. Enriquez; R. G. Oliveira; J. F. Floriano; J. P.C. Marcondes; Et al.

doi:10.1371/journal.pone.0231096

Alterations in the structural characteristics of rectus abdominis muscles caused by diabetes and pregnancy: A comparative study of the rat model and women

Giovana Vesentini^*, Angélica M.P. Barbosa, Débora C. Damasceno, Gabriela Marini, Fernanda Piculo, Selma M.M. Matheus, Raghavendra L.S. Hallur, Sthefanie K. Nunes, Bruna B. Catinelli, Claudia G. Magalhães, Roberto Costa, Joelcio F. Abbade, José E. Corrente, Iracema M.P. Calderon, Marilza V.C. Rudge, F. P. Souza, A. Hijas, L. Sobrevia, M. S. Palma, C. F.O. GraeffR. K. Arni, R. D. Herculano, D. F. Salvadori, M. Dal Pai Silva, S. A.M. Lima, C. Emanueli, S. Felisbino, W. Barbosa, A. Atallah, M. J.B. Girão, Z. Di Bella, S. M. Uchoa, S. Payão, B. Berghman, R. De Bie, B. Junginger, F. C.B. Alves, P. S. Rossignoli, C. B. Prudencio, M. I.G. Orlandi, M. I. Gonçalves, B. V. Sarmento, F. A. Pinheiro, C. I. Sartorão, S. B.C.V. Quiroz, D. R.A. Reyes, E. M.A. Enriquez, R. G. Oliveira, J. F. Floriano, J. P.C. Marcondes, Et al.

^*Corresponding author for this work

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Abstract

Background and objective In the present study, we compared the effect of diabetic pregnancy on the rectus abdominis muscle (RAM) in humans and rats. We hypothesized that our animal model could provide valuable information about alterations in the RAM of women with Gestational Diabetes (GDM). Method Newborns female rats (n = 10/group) were administered streptozotocin (100 mg/kg body weight) subcutaneously and were mated on reaching adulthood, to develop the mild hyperglycemic pregnant (MHP) rat model. At the end of pregnancy, the mothers were sacrificed, and the RAM tissue was collected. Pregnant women without GDM (non-GDM group; n = 10) and those diagnosed with GDM (GDM group; n = 8) and undergoing treatment were recruited, and RAM samples were obtained at C-section. The RAM architecture and the distribution of the fast and slow fibers and collagen were studied by immunohistochemistry. Results No statistically significant differences in the maternal and fetal characters were observed between the groups in both rats and women. However, significant changes in RAM architecture were observed. Diabetes in pregnancy increased the abundance of slow fibers and decreased fast fiber number and area in both rats and women. A decrease in collagen distribution was observed in GDM women; however, a similar change was not observed in the MHP rats. Conclusion Our results indicated that pregnancy- associated diabetes- induced similar structural adaptations in the RAM of women and rats with slight alterations in fiber type number and area. These findings suggest that the MHP rat model can be used for studying the effects of pregnancy-associated diabetes on the fiber structure of RAM.

Original language	English
Article number	e0231096
Number of pages	17
Journal	PLOS ONE
Volume	15
Issue number	4
DOIs	https://doi.org/10.1371/journal.pone.0231096
Publication status	Published - 3 Apr 2020

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Vesentini, G., Barbosa, A. M. P., Damasceno, D. C., Marini, G., Piculo, F., Matheus, S. M. M., Hallur, R. L. S., Nunes, S. K., Catinelli, B. B., Magalhães, C. G., Costa, R., Abbade, J. F., Corrente, J. E., Calderon, I. M. P., Rudge, M. V. C., Souza, F. P., Hijas, A., Sobrevia, L., Palma, M. S., ... Et al. (2020). Alterations in the structural characteristics of rectus abdominis muscles caused by diabetes and pregnancy: A comparative study of the rat model and women. PLOS ONE, 15(4), Article e0231096. https://doi.org/10.1371/journal.pone.0231096

@article{fff3febfe87543bebad59e3bfbdc4dd2,

title = "Alterations in the structural characteristics of rectus abdominis muscles caused by diabetes and pregnancy: A comparative study of the rat model and women",

abstract = "Background and objective In the present study, we compared the effect of diabetic pregnancy on the rectus abdominis muscle (RAM) in humans and rats. We hypothesized that our animal model could provide valuable information about alterations in the RAM of women with Gestational Diabetes (GDM). Method Newborns female rats (n = 10/group) were administered streptozotocin (100 mg/kg body weight) subcutaneously and were mated on reaching adulthood, to develop the mild hyperglycemic pregnant (MHP) rat model. At the end of pregnancy, the mothers were sacrificed, and the RAM tissue was collected. Pregnant women without GDM (non-GDM group; n = 10) and those diagnosed with GDM (GDM group; n = 8) and undergoing treatment were recruited, and RAM samples were obtained at C-section. The RAM architecture and the distribution of the fast and slow fibers and collagen were studied by immunohistochemistry. Results No statistically significant differences in the maternal and fetal characters were observed between the groups in both rats and women. However, significant changes in RAM architecture were observed. Diabetes in pregnancy increased the abundance of slow fibers and decreased fast fiber number and area in both rats and women. A decrease in collagen distribution was observed in GDM women; however, a similar change was not observed in the MHP rats. Conclusion Our results indicated that pregnancy- associated diabetes- induced similar structural adaptations in the RAM of women and rats with slight alterations in fiber type number and area. These findings suggest that the MHP rat model can be used for studying the effects of pregnancy-associated diabetes on the fiber structure of RAM.",

author = "Giovana Vesentini and Barbosa, {Ang{\'e}lica M.P.} and Damasceno, {D{\'e}bora C.} and Gabriela Marini and Fernanda Piculo and Matheus, {Selma M.M.} and Hallur, {Raghavendra L.S.} and Nunes, {Sthefanie K.} and Catinelli, {Bruna B.} and Magalh{\~a}es, {Claudia G.} and Roberto Costa and Abbade, {Joelcio F.} and Corrente, {Jos{\'e} E.} and Calderon, {Iracema M.P.} and Rudge, {Marilza V.C.} and Souza, {F. P.} and A. Hijas and L. Sobrevia and Palma, {M. S.} and Graeff, {C. F.O.} and Arni, {R. K.} and Herculano, {R. D.} and Salvadori, {D. F.} and {Dal Pai Silva}, M. and Lima, {S. A.M.} and C. Emanueli and S. Felisbino and W. Barbosa and A. Atallah and Gir{\~a}o, {M. J.B.} and {Di Bella}, Z. and Uchoa, {S. M.} and S. Pay{\~a}o and B. Berghman and {De Bie}, R. and B. Junginger and Alves, {F. C.B.} and Rossignoli, {P. S.} and Prudencio, {C. B.} and Orlandi, {M. I.G.} and Gon{\c c}alves, {M. I.} and Sarmento, {B. V.} and Pinheiro, {F. A.} and Sartor{\~a}o, {C. I.} and Quiroz, {S. B.C.V.} and Reyes, {D. R.A.} and Enriquez, {E. M.A.} and Oliveira, {R. G.} and Floriano, {J. F.} and Marcondes, {J. P.C.} and {Et al.}",

note = "Funding Information: The financial support provided by the Brazilian Public Health System (SUS) supported the treatment of all pregnant women. The authors gratefully acknowledge funding by S{\~a}o Paulo Research Foundation (FAPESP) (Grant No: #2016/09710-9 and #2016/01743-5). GV received a FAPESP scholarship (Grant No: #2014/26852-6). Funding Information: Funding:Thefinancialsupportprovidedbythe BrazilianPublicHealthSystem(SUS)supported thetreatmentofallpregnantwomen.Theauthors gratefullyacknowledgefundingbyS{\~a}oPaulo ResearchFoundation(FAPESP)(GrantNo:#2016/ 09710-9and#2016/01743-5).GVreceiveda FAPESPscholarship(GrantNo:#2014/26852-6). Publisher Copyright: {\textcopyright} 2020 Vesentini et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",

year = "2020",

month = apr,

day = "3",

doi = "10.1371/journal.pone.0231096",

language = "English",

volume = "15",

journal = "PLOS ONE",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "4",

}

Vesentini, G, Barbosa, AMP, Damasceno, DC, Marini, G, Piculo, F, Matheus, SMM, Hallur, RLS, Nunes, SK, Catinelli, BB, Magalhães, CG, Costa, R, Abbade, JF, Corrente, JE, Calderon, IMP, Rudge, MVC, Souza, FP, Hijas, A, Sobrevia, L, Palma, MS, Graeff, CFO, Arni, RK, Herculano, RD, Salvadori, DF, Dal Pai Silva, M, Lima, SAM, Emanueli, C, Felisbino, S, Barbosa, W, Atallah, A, Girão, MJB, Di Bella, Z, Uchoa, SM, Payão, S, Berghman, B, De Bie, R, Junginger, B, Alves, FCB, Rossignoli, PS, Prudencio, CB, Orlandi, MIG, Gonçalves, MI, Sarmento, BV, Pinheiro, FA, Sartorão, CI, Quiroz, SBCV, Reyes, DRA, Enriquez, EMA, Oliveira, RG, Floriano, JF, Marcondes, JPC & Et al. 2020, 'Alterations in the structural characteristics of rectus abdominis muscles caused by diabetes and pregnancy: A comparative study of the rat model and women', PLOS ONE, vol. 15, no. 4, e0231096. https://doi.org/10.1371/journal.pone.0231096

TY - JOUR

T1 - Alterations in the structural characteristics of rectus abdominis muscles caused by diabetes and pregnancy

T2 - A comparative study of the rat model and women

AU - Vesentini, Giovana

AU - Barbosa, Angélica M.P.

AU - Damasceno, Débora C.

AU - Marini, Gabriela

AU - Piculo, Fernanda

AU - Matheus, Selma M.M.

AU - Hallur, Raghavendra L.S.

AU - Nunes, Sthefanie K.

AU - Catinelli, Bruna B.

AU - Magalhães, Claudia G.

AU - Costa, Roberto

AU - Abbade, Joelcio F.

AU - Corrente, José E.

AU - Calderon, Iracema M.P.

AU - Rudge, Marilza V.C.

AU - Souza, F. P.

AU - Hijas, A.

AU - Sobrevia, L.

AU - Palma, M. S.

AU - Graeff, C. F.O.

AU - Arni, R. K.

AU - Herculano, R. D.

AU - Salvadori, D. F.

AU - Dal Pai Silva, M.

AU - Lima, S. A.M.

AU - Emanueli, C.

AU - Felisbino, S.

AU - Barbosa, W.

AU - Atallah, A.

AU - Girão, M. J.B.

AU - Di Bella, Z.

AU - Uchoa, S. M.

AU - Payão, S.

AU - Berghman, B.

AU - De Bie, R.

AU - Junginger, B.

AU - Alves, F. C.B.

AU - Rossignoli, P. S.

AU - Prudencio, C. B.

AU - Orlandi, M. I.G.

AU - Gonçalves, M. I.

AU - Sarmento, B. V.

AU - Pinheiro, F. A.

AU - Sartorão, C. I.

AU - Quiroz, S. B.C.V.

AU - Reyes, D. R.A.

AU - Enriquez, E. M.A.

AU - Oliveira, R. G.

AU - Floriano, J. F.

AU - Marcondes, J. P.C.

AU - Et al.

N1 - Funding Information: The financial support provided by the Brazilian Public Health System (SUS) supported the treatment of all pregnant women. The authors gratefully acknowledge funding by São Paulo Research Foundation (FAPESP) (Grant No: #2016/09710-9 and #2016/01743-5). GV received a FAPESP scholarship (Grant No: #2014/26852-6). Funding Information: Funding:Thefinancialsupportprovidedbythe BrazilianPublicHealthSystem(SUS)supported thetreatmentofallpregnantwomen.Theauthors gratefullyacknowledgefundingbySãoPaulo ResearchFoundation(FAPESP)(GrantNo:#2016/ 09710-9and#2016/01743-5).GVreceiveda FAPESPscholarship(GrantNo:#2014/26852-6). Publisher Copyright: © 2020 Vesentini et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2020/4/3

Y1 - 2020/4/3

N2 - Background and objective In the present study, we compared the effect of diabetic pregnancy on the rectus abdominis muscle (RAM) in humans and rats. We hypothesized that our animal model could provide valuable information about alterations in the RAM of women with Gestational Diabetes (GDM). Method Newborns female rats (n = 10/group) were administered streptozotocin (100 mg/kg body weight) subcutaneously and were mated on reaching adulthood, to develop the mild hyperglycemic pregnant (MHP) rat model. At the end of pregnancy, the mothers were sacrificed, and the RAM tissue was collected. Pregnant women without GDM (non-GDM group; n = 10) and those diagnosed with GDM (GDM group; n = 8) and undergoing treatment were recruited, and RAM samples were obtained at C-section. The RAM architecture and the distribution of the fast and slow fibers and collagen were studied by immunohistochemistry. Results No statistically significant differences in the maternal and fetal characters were observed between the groups in both rats and women. However, significant changes in RAM architecture were observed. Diabetes in pregnancy increased the abundance of slow fibers and decreased fast fiber number and area in both rats and women. A decrease in collagen distribution was observed in GDM women; however, a similar change was not observed in the MHP rats. Conclusion Our results indicated that pregnancy- associated diabetes- induced similar structural adaptations in the RAM of women and rats with slight alterations in fiber type number and area. These findings suggest that the MHP rat model can be used for studying the effects of pregnancy-associated diabetes on the fiber structure of RAM.

AB - Background and objective In the present study, we compared the effect of diabetic pregnancy on the rectus abdominis muscle (RAM) in humans and rats. We hypothesized that our animal model could provide valuable information about alterations in the RAM of women with Gestational Diabetes (GDM). Method Newborns female rats (n = 10/group) were administered streptozotocin (100 mg/kg body weight) subcutaneously and were mated on reaching adulthood, to develop the mild hyperglycemic pregnant (MHP) rat model. At the end of pregnancy, the mothers were sacrificed, and the RAM tissue was collected. Pregnant women without GDM (non-GDM group; n = 10) and those diagnosed with GDM (GDM group; n = 8) and undergoing treatment were recruited, and RAM samples were obtained at C-section. The RAM architecture and the distribution of the fast and slow fibers and collagen were studied by immunohistochemistry. Results No statistically significant differences in the maternal and fetal characters were observed between the groups in both rats and women. However, significant changes in RAM architecture were observed. Diabetes in pregnancy increased the abundance of slow fibers and decreased fast fiber number and area in both rats and women. A decrease in collagen distribution was observed in GDM women; however, a similar change was not observed in the MHP rats. Conclusion Our results indicated that pregnancy- associated diabetes- induced similar structural adaptations in the RAM of women and rats with slight alterations in fiber type number and area. These findings suggest that the MHP rat model can be used for studying the effects of pregnancy-associated diabetes on the fiber structure of RAM.

U2 - 10.1371/journal.pone.0231096

DO - 10.1371/journal.pone.0231096

M3 - Article

SN - 1932-6203

VL - 15

JO - PLOS ONE

JF - PLOS ONE

IS - 4

M1 - e0231096

ER -

Alterations in the structural characteristics of rectus abdominis muscles caused by diabetes and pregnancy: A comparative study of the rat model and women

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