Alpha7 acetylcholine receptor autoantibodies are rare in sera of patients diagnosed with schizophrenia or bipolar disorder

Carolin Hoffmann; Jo Stevens; Shenghua Zong; Daan van Kruining; Abhishek Saxena; Cem Ismail Kucukali; Erdem Tuzun; Nazli Yalcinkaya; Marc De Hert; Emiliano Gonzalez-Vioque; Celso Arango; Jon Lindstrom; Marc H. De Beets; Bail P. F. Rutter; Jim van Os; Peter Molenaar; Mario Losen; Pilar Martinez-Martinez

doi:10.1371/journal.pone.0208412

Alpha7 acetylcholine receptor autoantibodies are rare in sera of patients diagnosed with schizophrenia or bipolar disorder

Carolin Hoffmann, Jo Stevens, Shenghua Zong, Daan van Kruining, Abhishek Saxena, Cem Ismail Kucukali, Erdem Tuzun, Nazli Yalcinkaya, Marc De Hert, Emiliano Gonzalez-Vioque, Celso Arango, Jon Lindstrom, Marc H. De Beets, Bail P. F. Rutter, Jim van Os, Peter Molenaar, Mario Losen^*, Pilar Martinez-Martinez^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The alpha 7 acetylcholine receptor (AChR) has been linked with the onset of psychotic symptoms and we hypothesized therefore that it might also be an autoimmune target. Here, we describe a new radioimmunoassay (RIA) using iodine 125-labelled alpha-bungarotoxin and membrane extract from transfected HEK293 cells expressing human alpha 7 AChR. This RIA was used to analyze sera pertaining to a cohort of 711 subjects, comprising 368 patients diagnosed with schizophrenia spectrum disorders, 140 with bipolar disorder, 58 individuals diagnosed of other mental disorders, and 118 healthy comparison subjects. We identified one patient whose serum tested positive although with very low levels (0.2 nM) for alpha 7 AChR-specific antibodies by RIA. Three out of 711 sera contained antibodies against iodine 125-labelled alpha-bungarotoxin, because they precipitated with it in the absence of alpha 7 AChR. This first evidence suggests that autoantibodies against alpha 7 AChR are absent or very rare in these clinical groups.

Original language	English
Article number	e0208412
Number of pages	12
Journal	PLOS ONE
Volume	13
Issue number	12
DOIs	https://doi.org/10.1371/journal.pone.0208412
Publication status	Published - 6 Dec 2018

Keywords

NICOTINIC RECEPTOR
MYASTHENIA-GRAVIS
ANTIBODY
SUBUNIT
RELEVANCE
SYSTEM
CORTEX
BRAIN

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Hoffmann, C., Stevens, J., Zong, S., van Kruining, D., Saxena, A., Kucukali, C. I., Tuzun, E., Yalcinkaya, N., De Hert, M., Gonzalez-Vioque, E., Arango, C., Lindstrom, J., De Beets, M. H., Rutter, B. P. F., van Os, J., Molenaar, P., Losen, M., & Martinez-Martinez, P. (2018). Alpha7 acetylcholine receptor autoantibodies are rare in sera of patients diagnosed with schizophrenia or bipolar disorder. PLOS ONE, 13(12), Article e0208412. https://doi.org/10.1371/journal.pone.0208412

@article{b96c72d600fc4f6c9f41ab1f0c589f21,

title = "Alpha7 acetylcholine receptor autoantibodies are rare in sera of patients diagnosed with schizophrenia or bipolar disorder",

abstract = "The alpha 7 acetylcholine receptor (AChR) has been linked with the onset of psychotic symptoms and we hypothesized therefore that it might also be an autoimmune target. Here, we describe a new radioimmunoassay (RIA) using iodine 125-labelled alpha-bungarotoxin and membrane extract from transfected HEK293 cells expressing human alpha 7 AChR. This RIA was used to analyze sera pertaining to a cohort of 711 subjects, comprising 368 patients diagnosed with schizophrenia spectrum disorders, 140 with bipolar disorder, 58 individuals diagnosed of other mental disorders, and 118 healthy comparison subjects. We identified one patient whose serum tested positive although with very low levels (0.2 nM) for alpha 7 AChR-specific antibodies by RIA. Three out of 711 sera contained antibodies against iodine 125-labelled alpha-bungarotoxin, because they precipitated with it in the absence of alpha 7 AChR. This first evidence suggests that autoantibodies against alpha 7 AChR are absent or very rare in these clinical groups.",

keywords = "NICOTINIC RECEPTOR, MYASTHENIA-GRAVIS, ANTIBODY, SUBUNIT, RELEVANCE, SYSTEM, CORTEX, BRAIN",

author = "Carolin Hoffmann and Jo Stevens and Shenghua Zong and {van Kruining}, Daan and Abhishek Saxena and Kucukali, {Cem Ismail} and Erdem Tuzun and Nazli Yalcinkaya and {De Hert}, Marc and Emiliano Gonzalez-Vioque and Celso Arango and Jon Lindstrom and {De Beets}, {Marc H.} and Rutter, {Bail P. F.} and {van Os}, Jim and Peter Molenaar and Mario Losen and Pilar Martinez-Martinez",

year = "2018",

month = dec,

day = "6",

doi = "10.1371/journal.pone.0208412",

language = "English",

volume = "13",

journal = "PLOS ONE",

issn = "1932-6203",

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Hoffmann, C, Stevens, J, Zong, S, van Kruining, D, Saxena, A, Kucukali, CI, Tuzun, E, Yalcinkaya, N, De Hert, M, Gonzalez-Vioque, E, Arango, C, Lindstrom, J, De Beets, MH, Rutter, BPF, van Os, J, Molenaar, P , Losen, M & Martinez-Martinez, P 2018, 'Alpha7 acetylcholine receptor autoantibodies are rare in sera of patients diagnosed with schizophrenia or bipolar disorder', PLOS ONE, vol. 13, no. 12, e0208412. https://doi.org/10.1371/journal.pone.0208412

TY - JOUR

T1 - Alpha7 acetylcholine receptor autoantibodies are rare in sera of patients diagnosed with schizophrenia or bipolar disorder

AU - Hoffmann, Carolin

AU - Stevens, Jo

AU - Zong, Shenghua

AU - van Kruining, Daan

AU - Saxena, Abhishek

AU - Kucukali, Cem Ismail

AU - Tuzun, Erdem

AU - Yalcinkaya, Nazli

AU - De Hert, Marc

AU - Gonzalez-Vioque, Emiliano

AU - Arango, Celso

AU - Lindstrom, Jon

AU - De Beets, Marc H.

AU - Rutter, Bail P. F.

AU - van Os, Jim

AU - Molenaar, Peter

AU - Losen, Mario

AU - Martinez-Martinez, Pilar

PY - 2018/12/6

Y1 - 2018/12/6

N2 - The alpha 7 acetylcholine receptor (AChR) has been linked with the onset of psychotic symptoms and we hypothesized therefore that it might also be an autoimmune target. Here, we describe a new radioimmunoassay (RIA) using iodine 125-labelled alpha-bungarotoxin and membrane extract from transfected HEK293 cells expressing human alpha 7 AChR. This RIA was used to analyze sera pertaining to a cohort of 711 subjects, comprising 368 patients diagnosed with schizophrenia spectrum disorders, 140 with bipolar disorder, 58 individuals diagnosed of other mental disorders, and 118 healthy comparison subjects. We identified one patient whose serum tested positive although with very low levels (0.2 nM) for alpha 7 AChR-specific antibodies by RIA. Three out of 711 sera contained antibodies against iodine 125-labelled alpha-bungarotoxin, because they precipitated with it in the absence of alpha 7 AChR. This first evidence suggests that autoantibodies against alpha 7 AChR are absent or very rare in these clinical groups.

AB - The alpha 7 acetylcholine receptor (AChR) has been linked with the onset of psychotic symptoms and we hypothesized therefore that it might also be an autoimmune target. Here, we describe a new radioimmunoassay (RIA) using iodine 125-labelled alpha-bungarotoxin and membrane extract from transfected HEK293 cells expressing human alpha 7 AChR. This RIA was used to analyze sera pertaining to a cohort of 711 subjects, comprising 368 patients diagnosed with schizophrenia spectrum disorders, 140 with bipolar disorder, 58 individuals diagnosed of other mental disorders, and 118 healthy comparison subjects. We identified one patient whose serum tested positive although with very low levels (0.2 nM) for alpha 7 AChR-specific antibodies by RIA. Three out of 711 sera contained antibodies against iodine 125-labelled alpha-bungarotoxin, because they precipitated with it in the absence of alpha 7 AChR. This first evidence suggests that autoantibodies against alpha 7 AChR are absent or very rare in these clinical groups.

KW - NICOTINIC RECEPTOR

KW - MYASTHENIA-GRAVIS

KW - ANTIBODY

KW - SUBUNIT

KW - RELEVANCE

KW - SYSTEM

KW - CORTEX

KW - BRAIN

U2 - 10.1371/journal.pone.0208412

DO - 10.1371/journal.pone.0208412

M3 - Article

C2 - 30521579

SN - 1932-6203

VL - 13

JO - PLOS ONE

JF - PLOS ONE

IS - 12

M1 - e0208412

ER -

Alpha7 acetylcholine receptor autoantibodies are rare in sera of patients diagnosed with schizophrenia or bipolar disorder

Abstract

Keywords

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