Advancing the cell culture landscape: the instructive potential of artificial and natural geometries

Research output: ThesisDoctoral ThesisInternal

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Abstract

This research focuses on how surface structures can influence the behaviour of cells. There is a great diversity of surface structures, which makes the identification of an optimal physical environment for a specific phenotype difficult. Therefore, platforms that allow screening of many different designs at the same time facilitate the identification of an optimal cultural environment. Using the TopoChip, which contains 2176 unique microtopographies, structures have been identified that support the tenocyte phenotype, the primary cell type of the tendon. In addition, this also applies to mesenchymal stem cells (MSCs), which experience an activation of tendon-related genes. Furthermore, the library has been creatively expanded by using natural surface topographies that cause unique cell behaviour, such as promoting osteogenesis.
Original languageEnglish
Awarding Institution
  • Maastricht University
Supervisors/Advisors
  • de Boer, Jan, Supervisor, External person
  • Carlier, Aurélie, Co-Supervisor
Award date4 Sep 2020
Place of PublicationMaastricht
Publisher
Print ISBNs9789463808866
DOIs
Publication statusPublished - 2020

Keywords

  • cell culture
  • physical cues
  • mechanobiology
  • cell phenotype
  • regenerative medicine

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