@inbook{da77e73e1d594e3c8ccd4cadcb89b930,
title = "Adipogenesis from bovine precursors",
abstract = "Cultured meat, or tissue engineered meat, is a promising alternative to conventional meat production. In order to realistically mimic the multiple tissue types found in beef, food-compatible methods for bovine fat tissue engineering must be developed. We present a protocol for the isolation of adipose tissue-derived preadipocytes and subsequent adipogenic differentiation through free fatty acid stimulation. Differentiating preadipocytes can be either grown in 2D culture conditions or seeded in 3D alginate scaffolds. Differentiation is visually confirmed through lipophilic staining.",
keywords = "Adipogenic differentiation, Adipose, Alginate, Bovine, Branched chain fatty acid, Cultured meat, Free fatty acid, Lipid, Monounsaturated fatty acid, Preadipocyte",
author = "Frea Mehta and Ruud Theunissen and Post, {Mark J.}",
note = "Funding Information: This work was supported by the Brin-Wojcicki Foundation, the Fulbright U.S. Student Program, and the Netherland-America Foundation. Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-8897-6_8",
language = "English",
isbn = "978-1-4939-8896-9",
volume = "1889",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "111--125",
editor = "R{\o}nning, {Sissel Beate}",
booktitle = "Myogenesis",
address = "United States",
}