Adipocyte calcium sensing receptor is not involved in visceral adipose tissue inflammation or atherosclerosis development in hyperlipidemic Apoe(-/-) mice

Sai Sahana Sundararaman; Linsey J. F. Peters; Yvonne Jansen; Selin Gencer; Yi Yan; Sumra Nazir; Andrea Bonnin Marquez; Florian Kahles; Michael Lehrke; Erik A. L. Biessen; Joachim Jankowski; Christian Weber; Yvonne Doering; Emiel P. C. van der Vorst

doi:10.1038/s41598-021-89893-y

Adipocyte calcium sensing receptor is not involved in visceral adipose tissue inflammation or atherosclerosis development in hyperlipidemic Apoe(-/-) mice

Sai Sahana Sundararaman, Linsey J. F. Peters, Yvonne Jansen, Selin Gencer, Yi Yan, Sumra Nazir, Andrea Bonnin Marquez, Florian Kahles, Michael Lehrke, Erik A. L. Biessen, Joachim Jankowski, Christian Weber, Yvonne Doering, Emiel P. C. van der Vorst^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The calcium sensing receptor (CaSR) is a G-protein coupled receptor that especially plays an important role in the sensing of extracellular calcium to maintain its homeostasis. Several in-vitro studies demonstrated that CaSR plays a role in adipose tissue metabolism and inflammation, resulting in systemic inflammation and contributing to atherosclerosis development. The aim of this study was to investigate whether adipocyte CaSR plays a role in adipose tissue inflammation in-vivo and atherosclerosis development. By using a newly established conditional mature adipocyte specific CaSR deficient mouse on a hyperlipidemic and atherosclerosis prone Apoe(-/-) background it could be shown that CaSR deficiency in adipocytes does neither contribute to initiation nor to progression of atherosclerotic plaques as judged by the unchanged lesion size or composition. Additionally, CaSR deficiency did not influence gonadal visceral adipose tissue (vAT) inflammation in-vivo, although a small decrease in gonadal visceral adipose cholesterol content could be observed. In conclusion, adipocyte CaSR seems not to be involved in vAT inflammation in-vivo and does not influence atherosclerosis development in hyperlipidemic Apoe(-/-) mice.

Original language	English
Article number	10409
Number of pages	9
Journal	Scientific Reports
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41598-021-89893-y
Publication status	Published - 17 May 2021

Keywords

TUMOR-NECROSIS-FACTOR
EXTRACELLULAR CA2+-SENSING RECEPTOR
INTERLEUKIN-6 PRODUCTION
PARATHYROID-HORMONE
KAPPA-B
EXPRESSION
ACTIVATION
OBESITY
PROLIFERATION
DISEASE

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Sundararaman, S. S., Peters, L. J. F., Jansen, Y., Gencer, S., Yan, Y., Nazir, S., Marquez, A. B., Kahles, F., Lehrke, M., Biessen, E. A. L., Jankowski, J., Weber, C., Doering, Y., & van der Vorst, E. P. C. (2021). Adipocyte calcium sensing receptor is not involved in visceral adipose tissue inflammation or atherosclerosis development in hyperlipidemic Apoe(-/-) mice. Scientific Reports, 11(1), Article 10409. https://doi.org/10.1038/s41598-021-89893-y

@article{c21c89b3a38c4fe3afa650f03b196586,

title = "Adipocyte calcium sensing receptor is not involved in visceral adipose tissue inflammation or atherosclerosis development in hyperlipidemic Apoe(-/-) mice",

abstract = "The calcium sensing receptor (CaSR) is a G-protein coupled receptor that especially plays an important role in the sensing of extracellular calcium to maintain its homeostasis. Several in-vitro studies demonstrated that CaSR plays a role in adipose tissue metabolism and inflammation, resulting in systemic inflammation and contributing to atherosclerosis development. The aim of this study was to investigate whether adipocyte CaSR plays a role in adipose tissue inflammation in-vivo and atherosclerosis development. By using a newly established conditional mature adipocyte specific CaSR deficient mouse on a hyperlipidemic and atherosclerosis prone Apoe(-/-) background it could be shown that CaSR deficiency in adipocytes does neither contribute to initiation nor to progression of atherosclerotic plaques as judged by the unchanged lesion size or composition. Additionally, CaSR deficiency did not influence gonadal visceral adipose tissue (vAT) inflammation in-vivo, although a small decrease in gonadal visceral adipose cholesterol content could be observed. In conclusion, adipocyte CaSR seems not to be involved in vAT inflammation in-vivo and does not influence atherosclerosis development in hyperlipidemic Apoe(-/-) mice.",

keywords = "TUMOR-NECROSIS-FACTOR, EXTRACELLULAR CA2+-SENSING RECEPTOR, INTERLEUKIN-6 PRODUCTION, PARATHYROID-HORMONE, KAPPA-B, EXPRESSION, ACTIVATION, OBESITY, PROLIFERATION, DISEASE",

author = "Sundararaman, {Sai Sahana} and Peters, {Linsey J. F.} and Yvonne Jansen and Selin Gencer and Yi Yan and Sumra Nazir and Marquez, {Andrea Bonnin} and Florian Kahles and Michael Lehrke and Biessen, {Erik A. L.} and Joachim Jankowski and Christian Weber and Yvonne Doering and {van der Vorst}, {Emiel P. C.}",

note = "Funding Information: Open Access funding enabled and organized by Projekt DEAL. The authors{\textquoteright} research is supported by the European Research Council AdG°692511 and German Centre for Cardiovascular Research (DZHK;81Z0600202) to C.W; by the Deutsche Forschungsgemeinschaft to JJ (SFB TRR219, C-03) and ML (SFB TRR219, M-03); by the Alexander von Humboldt Foundation, a Grant from the Interdisciplinary Center for Clinical Research within the faculty of Medicine at the RWTH Aachen University, the DZHK (German Centre for Cardiovascular Research) and the BMBF (German Ministry of Education and Research), and NWO-ZonMw Veni (91619053) to E.P.C.v.d.V. Publisher Copyright: {\textcopyright} 2021, The Author(s).",

year = "2021",

month = may,

day = "17",

doi = "10.1038/s41598-021-89893-y",

language = "English",

volume = "11",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

number = "1",

}

Sundararaman, SS, Peters, LJF, Jansen, Y, Gencer, S, Yan, Y, Nazir, S, Marquez, AB, Kahles, F, Lehrke, M, Biessen, EAL , Jankowski, J , Weber, C, Doering, Y & van der Vorst, EPC 2021, 'Adipocyte calcium sensing receptor is not involved in visceral adipose tissue inflammation or atherosclerosis development in hyperlipidemic Apoe(-/-) mice', Scientific Reports, vol. 11, no. 1, 10409. https://doi.org/10.1038/s41598-021-89893-y

TY - JOUR

T1 - Adipocyte calcium sensing receptor is not involved in visceral adipose tissue inflammation or atherosclerosis development in hyperlipidemic Apoe(-/-) mice

AU - Sundararaman, Sai Sahana

AU - Peters, Linsey J. F.

AU - Jansen, Yvonne

AU - Gencer, Selin

AU - Yan, Yi

AU - Nazir, Sumra

AU - Marquez, Andrea Bonnin

AU - Kahles, Florian

AU - Lehrke, Michael

AU - Biessen, Erik A. L.

AU - Jankowski, Joachim

AU - Weber, Christian

AU - Doering, Yvonne

AU - van der Vorst, Emiel P. C.

N1 - Funding Information: Open Access funding enabled and organized by Projekt DEAL. The authors’ research is supported by the European Research Council AdG°692511 and German Centre for Cardiovascular Research (DZHK;81Z0600202) to C.W; by the Deutsche Forschungsgemeinschaft to JJ (SFB TRR219, C-03) and ML (SFB TRR219, M-03); by the Alexander von Humboldt Foundation, a Grant from the Interdisciplinary Center for Clinical Research within the faculty of Medicine at the RWTH Aachen University, the DZHK (German Centre for Cardiovascular Research) and the BMBF (German Ministry of Education and Research), and NWO-ZonMw Veni (91619053) to E.P.C.v.d.V. Publisher Copyright: © 2021, The Author(s).

PY - 2021/5/17

Y1 - 2021/5/17

N2 - The calcium sensing receptor (CaSR) is a G-protein coupled receptor that especially plays an important role in the sensing of extracellular calcium to maintain its homeostasis. Several in-vitro studies demonstrated that CaSR plays a role in adipose tissue metabolism and inflammation, resulting in systemic inflammation and contributing to atherosclerosis development. The aim of this study was to investigate whether adipocyte CaSR plays a role in adipose tissue inflammation in-vivo and atherosclerosis development. By using a newly established conditional mature adipocyte specific CaSR deficient mouse on a hyperlipidemic and atherosclerosis prone Apoe(-/-) background it could be shown that CaSR deficiency in adipocytes does neither contribute to initiation nor to progression of atherosclerotic plaques as judged by the unchanged lesion size or composition. Additionally, CaSR deficiency did not influence gonadal visceral adipose tissue (vAT) inflammation in-vivo, although a small decrease in gonadal visceral adipose cholesterol content could be observed. In conclusion, adipocyte CaSR seems not to be involved in vAT inflammation in-vivo and does not influence atherosclerosis development in hyperlipidemic Apoe(-/-) mice.

AB - The calcium sensing receptor (CaSR) is a G-protein coupled receptor that especially plays an important role in the sensing of extracellular calcium to maintain its homeostasis. Several in-vitro studies demonstrated that CaSR plays a role in adipose tissue metabolism and inflammation, resulting in systemic inflammation and contributing to atherosclerosis development. The aim of this study was to investigate whether adipocyte CaSR plays a role in adipose tissue inflammation in-vivo and atherosclerosis development. By using a newly established conditional mature adipocyte specific CaSR deficient mouse on a hyperlipidemic and atherosclerosis prone Apoe(-/-) background it could be shown that CaSR deficiency in adipocytes does neither contribute to initiation nor to progression of atherosclerotic plaques as judged by the unchanged lesion size or composition. Additionally, CaSR deficiency did not influence gonadal visceral adipose tissue (vAT) inflammation in-vivo, although a small decrease in gonadal visceral adipose cholesterol content could be observed. In conclusion, adipocyte CaSR seems not to be involved in vAT inflammation in-vivo and does not influence atherosclerosis development in hyperlipidemic Apoe(-/-) mice.

KW - TUMOR-NECROSIS-FACTOR

KW - EXTRACELLULAR CA2+-SENSING RECEPTOR

KW - INTERLEUKIN-6 PRODUCTION

KW - PARATHYROID-HORMONE

KW - KAPPA-B

KW - EXPRESSION

KW - ACTIVATION

KW - OBESITY

KW - PROLIFERATION

KW - DISEASE

U2 - 10.1038/s41598-021-89893-y

DO - 10.1038/s41598-021-89893-y

M3 - Article

C2 - 34001955

SN - 2045-2322

VL - 11

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 10409

ER -

Adipocyte calcium sensing receptor is not involved in visceral adipose tissue inflammation or atherosclerosis development in hyperlipidemic Apoe(-/-) mice

Abstract

Keywords

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