Adenosine inhibits cytokine release and expression of adhesion molecules by activated human endothelial cells.

M.A. Bouma, F.A.J.M. van den Wildenberg, W.A. Buurman

Research output: Contribution to journalArticleAcademicpeer-review

134 Citations (Scopus)

Abstract

Adenosine inhibits cytokine release and expression of adhesion molecules by activated human endothelial cells.

Bouma MG, van den Wildenberg FA, Buurman WA.

Department of Surgery, University of Limburg, Maastricht, The Netherlands.

Ischemia induces excessive ATP catabolism with subsequent local release of its metabolite adenosine, an autacoid with anti-inflammatory properties. Because activation of the vascular endothelium is critical to the inflammatory host response during ischemia and reperfusion, the effects of adenosine on two major determinants of endothelial cell activation (i.e., the release of proinflammatory cytokines and the expression of adhesion molecules) were studied. Adenosine dose dependently inhibited the release of interleukin (IL)-6 and IL-8 by stimulated human umbilical vein endothelial cells (HUVEC). Expression of E-selectin and vascular cell adhesion molecule 1 (VCAM-1), but not intercellular adhesion molecule 1 (ICAM-1), by activated HUVEC was also reduced by adenosine. Inhibition of endogenous adenosine deaminase activity by erythro-9-(2-hydroxy-3-nonyl)adenine or 2'-deoxycoformycin strongly enhanced the inhibitory effects of exogenous adenosine on cytokine release and expression of E-selectin and VCAM-1. However, a clear role for specific adenosine receptors in the described inhibitory events could not be established. Together, these data imply that the vascular endothelium constitutes an important target for the anti-inflammatory actions of adenosine.
Original languageEnglish
Pages (from-to)C522-C529
JournalAmerican Journal of Physiology (Consolidated)
Volume270
DOIs
Publication statusPublished - 1 Jan 1996

Cite this