Background/objectives:Plant stanol esters lower serum low-density (LDL)-cholesterol (LDL-C), but responses between individuals vary ability of subjects to respond to acute dietary challenges may reflect flexibility to adapt to changes on the longer term, we related subjects' postprandial metabolic changes to changes in serum lipoproteins after intake of plant stanol esters.Subjects/methods:In a double-blind design, 20 healthy subjects received in random order a high-fat shake with or without plant stanol esters (4 g). Blood samples were taken examine lipid, glucose and lipoprotein profiles. Two subjects dropped the 3 weeks after this postprandial test, the subjects who received the with plant stanol esters continued the consumption of plant stanol- (3g/day) margarine and subjects receiving the control shake in the test consumed for the next 3 weeks a control margarine. After the subjects received the other shake and margarines.Results:The margarine with plant stanol esters lowered concentrations of total cholesterol by (P<0.01), LDL-C by 9.5% (P<0.01) and apoB100 by 8.6% (P<0.01). particle concentrations of total very low-density lipoprotein (VLDL), and large LDL were reduced by 26.6% (P=0.02), 27.6% (P=0.02) and 12.3% respectively. Plant stanol esters did not affect parameters related to glucose metabolism during the postprandial phase. However, the under the curve (iAUC) of the postprandial glucose concentration after the control shake correlated positively with changes in fasting total cholesterol, LDL-C, apoB100, total VLDL, small VLDL and intermediate-density lipoprotein after 3 weeks.Conclusions:A single dose stanol esters does not change postprandial lipid and lipoprotein However, postprandial glucose responses may predict the effects of stanol ester consumption.European Journal of Clinical Nutrition advance publication, 1 October 2014; doi:10.1038/ejcn.2014.200.