TY - JOUR
T1 - Detection of high-risk human papillomavirus HPV by the novel AmpFire isothermal HPV assay among pregnant women in Pemba Island, Tanzania
AU - Juliana, Naomi Christine Angela
AU - Juma, Mohamed Hamad
AU - Heijmans, Roel
AU - Ouburg, Sander
AU - Ali, Said Mohammed
AU - Chauhan, Aishwarya Singh
AU - Pemba, Amanhi Biobank
AU - Sazawal, Sunil
AU - Morre, Servaas Antonie
AU - Deb, Saikat
AU - Ambrosino, Elena
N1 - Funding Information:
Atila Biosystems Inc. (California, USA) sponsored the study by providing the HPV kits for free.
Publisher Copyright:
© Naomi Christine Angela Juliana et al.
PY - 2020/10/27
Y1 - 2020/10/27
N2 - Introduction: human papillomavirus (HPV) is the most common sexually transmitted virus in the world. Prevalence of infection differs, with highest rates reported in sub-Saharan African, including the country of Tanzania. In pregnancy, the hormonal changes and immune changes seem to facilitate HPV persistence, increasing the cancer risk and the risk of vertical transmission towards the placenta and the fetus. The burden of HPV infection is still high despite multiple screening and detection test available. The AmpFire® HPV assay is a novel nucleic acid isothermal amplification with real-time fluorescence detection assay that can test simultaneously 15 high-risk HPV. This nested cohort study aims to contribute evidence on the prevalence of HPV infection and persistence across two time points among pregnant women in Pemba island, Tanzania. Methods: vaginal swabs that were previously collected during pregnancy were stored in eNAT buffer (n
1 =385 and n
2 =187) and were tested with AmpFire® screening assay, for simultaneous detection of the HPV 16, 18 and other high-risk HPV genotypes 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66 and 68. Results: the AmpFire® HPV assay detected an 11% and 6% high-risk HPV prevalence at the two time points among pregnant women in Pemba island, consecutively. For the 133 women whose samples were tested at both time points, the persistence rate of high-risk HPV was 64%. Conclusion: novel isothermal HPV assay, such as the AmpFire®, might be feasible to use in low-income regions.
AB - Introduction: human papillomavirus (HPV) is the most common sexually transmitted virus in the world. Prevalence of infection differs, with highest rates reported in sub-Saharan African, including the country of Tanzania. In pregnancy, the hormonal changes and immune changes seem to facilitate HPV persistence, increasing the cancer risk and the risk of vertical transmission towards the placenta and the fetus. The burden of HPV infection is still high despite multiple screening and detection test available. The AmpFire® HPV assay is a novel nucleic acid isothermal amplification with real-time fluorescence detection assay that can test simultaneously 15 high-risk HPV. This nested cohort study aims to contribute evidence on the prevalence of HPV infection and persistence across two time points among pregnant women in Pemba island, Tanzania. Methods: vaginal swabs that were previously collected during pregnancy were stored in eNAT buffer (n
1 =385 and n
2 =187) and were tested with AmpFire® screening assay, for simultaneous detection of the HPV 16, 18 and other high-risk HPV genotypes 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66 and 68. Results: the AmpFire® HPV assay detected an 11% and 6% high-risk HPV prevalence at the two time points among pregnant women in Pemba island, consecutively. For the 133 women whose samples were tested at both time points, the persistence rate of high-risk HPV was 64%. Conclusion: novel isothermal HPV assay, such as the AmpFire®, might be feasible to use in low-income regions.
KW - HPV infection
KW - pregnancy
KW - AmpFire HPV
KW - isothermal
KW - sub-Saharan Africa
KW - HPV persistence
KW - PREVALENCE
KW - INFECTION
KW - CLEARANCE
U2 - 10.11604/pamj.2020.37.183.23367
DO - 10.11604/pamj.2020.37.183.23367
M3 - Article
C2 - 33447338
SN - 1937-8688
VL - 37
SP - 1
EP - 10
JO - Pan African Medical Journal
JF - Pan African Medical Journal
M1 - 183
ER -