A thermo-sensitive chitosan/pectin hydrogel for long-term tumor spheroid culture

G. Morello, A. Quarta, A. Gaballo, L. Moroni, G. Gigli, A. Polini*, F. Gervaso*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Hydrogels represent a key element in the development of in vitro tumor models, by mimicking the typical 3D tumor architecture in a physicochemical manner and allowing the study of tumor mechanisms. Here we developed a thermo-sensitive, natural polymer-based hydrogel, where chitosan and pectin were mixed and, after a weak base-induced chitosan gelation, a stable semi-Interpenetrating Polymer Network formed. This resulted thermo-responsive at 37 degrees C, injectable at room temperature, stable up to 6 weeks in vitro, permeable to small/ medium-sized molecules (3 to 70 kDa) and suitable for cell-encapsulation. Tunable mechanical and permeability properties were obtained by varying the polymer content. Optimized formulations successfully supported the formation and growth of human colorectal cancer spheroids up to 44 days of culture. The spheroid dimension and density were influenced by the semi-IPN stiffness and permeability. These encouraging results would allow the implementation of faithful tumor models for the study and development of personalized oncological treatments.
Original languageEnglish
Article number118633
Number of pages13
JournalCarbohydrate Polymers
Volume274
DOIs
Publication statusPublished - 15 Nov 2021

Keywords

  • Natural polymers
  • Thermo-responsive hydrogels
  • Semi-IPN
  • In vitro tumor models
  • Colorectal cancer
  • CRC spheroids
  • Tumor microenvironment
  • POLYELECTROLYTE COMPLEX
  • AQUEOUS MIXTURES
  • DRUG DISCOVERY
  • CELL-ADHESION
  • PECTIN
  • BEHAVIOR
  • GELATION
  • DELIVERY
  • SYSTEMS
  • CANCER

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