TY - JOUR
T1 - A synthesis approach of mouse studies to identify genes and proteins in arterial thrombosis and bleeding
AU - Baaten, Constance C. F. M. J.
AU - Meacham, Stuart
AU - de Witt, Susanne M.
AU - Feijge, Marion A. H.
AU - Adams, David J.
AU - Akkerman, Jan-Willem N.
AU - Cosemans, Judith M. E. M.
AU - Grassi, Luigi
AU - Jupe, Steve
AU - Kostadima, Myrto
AU - Mattheij, Nadine J. A.
AU - Prins, Martin H.
AU - Ramirez-Solis, Ramiro
AU - Soehnlein, Oliver
AU - Swieringa, Frauke
AU - Weber, Christian
AU - White, Jacqueline K.
AU - Ouwehand, Willem H.
AU - Heemskerk, Johan W. M.
N1 - © 2018 by The American Society of Hematology.
PY - 2018/12/13
Y1 - 2018/12/13
N2 - Antithrombotic therapies reduce cardiovascular diseases by preventing arterial thrombosis and thromboembolism, but at expense of increased bleeding risks. Arterial thrombosis studies using genetically modified mice have been invaluable for identification of new molecular targets. Because of low sample sizes and heterogeneity in approaches or methodologies, a formal meta-analysis to compare studies of mice with single-gene defects encountered major limitations. To overcome these, we developed a novel synthesis approach to quantitatively scale 1514 published studies of arterial thrombus formation (in vivo and in vitro), thromboembolism, and tail-bleeding of genetically modified mice. Using a newly defined consistency parameter (CP), indicating the strength of published data, comparisons were made of 431 mouse genes, of which 17 consistently contributed to thrombus formation without affecting hemostasis. Ranking analysis indicated high correlations between collagen-dependent thrombosis models in vivo (FeCl
3 injury or ligation/compression) and in vitro. Integration of scores and CP values resulted in a network of protein interactions in thrombosis and hemostasis (PITH), which was combined with databases of genetically linked human bleeding and thrombotic disorders. The network contained 2946 nodes linked to modifying genes of thrombus formation, mostly with expression in megakaryocytes. Reactome pathway analysis and network characteristics revealed multiple novel genes with potential contribution to thrombosis/hemostasis. Studies with additional knockout mice revealed that 4 of 8 (
Apoe,
Fpr2,
Ifnar1,
Vps13a) new genes were modifying in thrombus formation. The PITH network further: (i) revealed a high similarity of murine and human hemostatic and thrombotic processes and (ii) identified multiple new candidate proteins regulating these processes.
AB - Antithrombotic therapies reduce cardiovascular diseases by preventing arterial thrombosis and thromboembolism, but at expense of increased bleeding risks. Arterial thrombosis studies using genetically modified mice have been invaluable for identification of new molecular targets. Because of low sample sizes and heterogeneity in approaches or methodologies, a formal meta-analysis to compare studies of mice with single-gene defects encountered major limitations. To overcome these, we developed a novel synthesis approach to quantitatively scale 1514 published studies of arterial thrombus formation (in vivo and in vitro), thromboembolism, and tail-bleeding of genetically modified mice. Using a newly defined consistency parameter (CP), indicating the strength of published data, comparisons were made of 431 mouse genes, of which 17 consistently contributed to thrombus formation without affecting hemostasis. Ranking analysis indicated high correlations between collagen-dependent thrombosis models in vivo (FeCl
3 injury or ligation/compression) and in vitro. Integration of scores and CP values resulted in a network of protein interactions in thrombosis and hemostasis (PITH), which was combined with databases of genetically linked human bleeding and thrombotic disorders. The network contained 2946 nodes linked to modifying genes of thrombus formation, mostly with expression in megakaryocytes. Reactome pathway analysis and network characteristics revealed multiple novel genes with potential contribution to thrombosis/hemostasis. Studies with additional knockout mice revealed that 4 of 8 (
Apoe,
Fpr2,
Ifnar1,
Vps13a) new genes were modifying in thrombus formation. The PITH network further: (i) revealed a high similarity of murine and human hemostatic and thrombotic processes and (ii) identified multiple new candidate proteins regulating these processes.
KW - IN-VIVO
KW - PLATELET
KW - MECHANISMS
KW - SENSITIVITY
KW - DISORDERS
U2 - 10.1182/blood-2018-02-831982
DO - 10.1182/blood-2018-02-831982
M3 - Article
SN - 0006-4971
VL - 132
SP - E35-E46
JO - Blood
JF - Blood
IS - 24
ER -