A single dose of sodium nitrate does not improve oral glucose tolerance in patients with type 2 diabetes mellitus

Dietary nitrate (NO) supplementation has been proposed as an emerging treatment strategy for type 2 diabetes. We hypothesized that ingestion of a single bolus of dietary NO ingestion improves oral glucose tolerance in patients with type 2 diabetes. Seventeen men with type 2 diabetes (glycated hemoglobin, 7.3% +/- 0.2%) participated in a randomized crossover experiment. The subjects ingested a glucose beverage 2.5 hours after consumption of either sodium NO3- (0.15 mmol NaNO3- . kg(-1)) or a placebo solution. Venous blood samples were collected before ingestion of the glucose beverage and every 30 minutes thereafter during a 2-hour period to assess postprandial plasma glucose and insulin concentrations. The results show that plasma NO and nitrite levels were increased after NaNO3- as opposed to placebo ingestion (treatment-effect, P = .001). Despite the elevated plasma NO and nitrite levels, ingestion of NaNO3- did not attenuate the postprandial rise in plasma glucose and insulin concentrations (time x treatment interaction, P = .41 for glucose, P = .93 for insulin). Despite the lack of effect on oral glucose tolerance, basal plasma glucose concentrations measured 2.5 hours after NaNO3- ingestion were lower when compared with the placebo treatment (7.5 +/- 0.4 vs 8.3 +/- 0.4 mmol/L, respectively; P = .04). We conclude that ingestion of a single dose of dietary NO does not improve subsequent oral glucose tolerance in patients with type 2 diabetes.