TY - JOUR
T1 - A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in the absence of serum starvation
AU - Messmer, T.
AU - Klevernic, I.
AU - Furquim, C.
AU - Ovchinnikova, E.
AU - Dogan, A.
AU - Cruz, H.
AU - Post, M.J.
AU - Flack, J.E.
N1 - Funding Information:
T.M., I.K., C.F., E.O., A.D., H.C. and J.E.F. are employees of Mosa Meat BV. M.J.P. is co-founder and stakeholder of Mosa Meat BV. The study was funded by Mosa Meat BV. Mosa Meat BV has patents pending on serum-free proliferation medium (PCT/ P125933PC00) and serum-free differentiation medium (JBB/P126144NL00). All authors declare no other competing interests.
Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2022/1/1
Y1 - 2022/1/1
N2 - Cultured meat production requires the robust differentiation of satellite cells into mature muscle fibres without the use of animal-derived components. Current protocols induce myogenic differentiation in vitro through serum starvation, that is, an abrupt reduction in serum concentration. Here we used RNA sequencing to investigate the transcriptomic remodelling of bovine satellite cells during myogenic differentiation induced by serum starvation. We characterized canonical myogenic gene expression, and identified surface receptors upregulated during the early phase of differentiation, including IGF1R, TFRC and LPAR1. Supplementation of ligands to these receptors enabled the formulation of a chemically defined media that induced differentiation in the absence of serum starvation and/or transgene expression. Serum-free myogenic differentiation was of similar extent to that induced by serum starvation, as evaluated by transcriptome analysis, protein expression and the presence of a functional contractile apparatus. Moreover, the serum-free differentiation media supported the fabrication of three-dimensional bioartificial muscle constructs, demonstrating its suitability for cultured beef production.Bovine satellite cells undergoing myogenic differentiation in a chemically defined, serum-free medium are comparable to those undergoing serum starvation-demonstrating the suitability of this formulation for cultured meat production.
AB - Cultured meat production requires the robust differentiation of satellite cells into mature muscle fibres without the use of animal-derived components. Current protocols induce myogenic differentiation in vitro through serum starvation, that is, an abrupt reduction in serum concentration. Here we used RNA sequencing to investigate the transcriptomic remodelling of bovine satellite cells during myogenic differentiation induced by serum starvation. We characterized canonical myogenic gene expression, and identified surface receptors upregulated during the early phase of differentiation, including IGF1R, TFRC and LPAR1. Supplementation of ligands to these receptors enabled the formulation of a chemically defined media that induced differentiation in the absence of serum starvation and/or transgene expression. Serum-free myogenic differentiation was of similar extent to that induced by serum starvation, as evaluated by transcriptome analysis, protein expression and the presence of a functional contractile apparatus. Moreover, the serum-free differentiation media supported the fabrication of three-dimensional bioartificial muscle constructs, demonstrating its suitability for cultured beef production.Bovine satellite cells undergoing myogenic differentiation in a chemically defined, serum-free medium are comparable to those undergoing serum starvation-demonstrating the suitability of this formulation for cultured meat production.
KW - SKELETAL-MUSCLE DIFFERENTIATION
KW - IN-VITRO
KW - MYOGENIC DIFFERENTIATION
KW - REGULATORY CHALLENGES
KW - LYSOPHOSPHATIDIC ACID
KW - GROWTH-FACTORS
KW - MODEL
U2 - 10.1038/s43016-021-00419-1
DO - 10.1038/s43016-021-00419-1
M3 - Article
C2 - 37118488
SN - 2662-1355
VL - 3
SP - 74
EP - 85
JO - Nature food
JF - Nature food
IS - 1
ER -