A SELDI-TOF-MS Study in Lacunar Stroke with Subsequent Haptoglobin Phenotyping

J. Staals, J. Bons, R.J. van Oostenbrugge, I.L. Knottnerus, M.P. van Dieijen-Visser, F.G. Bouwman, E.C. Mariman, J.R. Delanghe, J. Lodder, W.K. Wodzig

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Using Surface-Enhanced Laser Desorption / Ionization Time-of-Flight Mass Spectrometry (SELDI-TOF-MS), we aimed to detect differences in protein profile in serum samples of two lacunar stroke subtypes. SELDI-TOF-MS, followed by protein identification, was performed in samples of 8 first-ever lacunar stroke patients with MR imaging showing a single symptomatic lacunar lesion (type 1), and 8 with multiple additional "silent" lacunar lesions and extensive white matter lesions (type 2). A 16 kDa protein, identified as alpha-2-chain of haptoglobin (Hp), was found to be overrepresented in type 1 compared to type 2 (peak intensity 12.5 vs. 5.0; p=0.02). As a polymorphism with two alleles, Hp-1 and Hp 2, determines the presence of alpha-1 and/or alpha-2-chains in the Hp-molecule, Hp phenotypic analysis was performed. Hp 1 : Hp-2 allele frequency was 0.562 : 0.438 in type 1 and 0.812 : 0.188 in type 2 (population reference approximately 0.4 : 0.6). We conclude that the overrepresentation of the alpha-2-chain in lacunar stroke type 1 compared to type 2 relates to a higher Hp-2 allele frequency in the former. Yet, compared to population reference, the phenotype distribution in both patient groups deviates towards a high Hp-1 allele frequency. Our findings suggest a role for the Hp gene in the etiology of cerebral small vessel disease. Larger studies are now needed to explore this new candidate gene.
Original languageEnglish
Pages (from-to)93-98
JournalCurrent Neurovascular Research
Volume5
Issue number2
DOIs
Publication statusPublished - 1 Jan 2008

Cite this

@article{9748d3491c894ec896eee0eaf5c73f4d,
title = "A SELDI-TOF-MS Study in Lacunar Stroke with Subsequent Haptoglobin Phenotyping",
abstract = "Using Surface-Enhanced Laser Desorption / Ionization Time-of-Flight Mass Spectrometry (SELDI-TOF-MS), we aimed to detect differences in protein profile in serum samples of two lacunar stroke subtypes. SELDI-TOF-MS, followed by protein identification, was performed in samples of 8 first-ever lacunar stroke patients with MR imaging showing a single symptomatic lacunar lesion (type 1), and 8 with multiple additional {"}silent{"} lacunar lesions and extensive white matter lesions (type 2). A 16 kDa protein, identified as alpha-2-chain of haptoglobin (Hp), was found to be overrepresented in type 1 compared to type 2 (peak intensity 12.5 vs. 5.0; p=0.02). As a polymorphism with two alleles, Hp-1 and Hp 2, determines the presence of alpha-1 and/or alpha-2-chains in the Hp-molecule, Hp phenotypic analysis was performed. Hp 1 : Hp-2 allele frequency was 0.562 : 0.438 in type 1 and 0.812 : 0.188 in type 2 (population reference approximately 0.4 : 0.6). We conclude that the overrepresentation of the alpha-2-chain in lacunar stroke type 1 compared to type 2 relates to a higher Hp-2 allele frequency in the former. Yet, compared to population reference, the phenotype distribution in both patient groups deviates towards a high Hp-1 allele frequency. Our findings suggest a role for the Hp gene in the etiology of cerebral small vessel disease. Larger studies are now needed to explore this new candidate gene.",
author = "J. Staals and J. Bons and {van Oostenbrugge}, R.J. and I.L. Knottnerus and {van Dieijen-Visser}, M.P. and F.G. Bouwman and E.C. Mariman and J.R. Delanghe and J. Lodder and W.K. Wodzig",
year = "2008",
month = "1",
day = "1",
doi = "10.2174/156720208784310187",
language = "English",
volume = "5",
pages = "93--98",
journal = "Current Neurovascular Research",
issn = "1567-2026",
publisher = "Bentham Science Publishers Ltd.",
number = "2",

}

A SELDI-TOF-MS Study in Lacunar Stroke with Subsequent Haptoglobin Phenotyping. / Staals, J.; Bons, J.; van Oostenbrugge, R.J.; Knottnerus, I.L.; van Dieijen-Visser, M.P.; Bouwman, F.G.; Mariman, E.C.; Delanghe, J.R.; Lodder, J.; Wodzig, W.K.

In: Current Neurovascular Research, Vol. 5, No. 2, 01.01.2008, p. 93-98.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - A SELDI-TOF-MS Study in Lacunar Stroke with Subsequent Haptoglobin Phenotyping

AU - Staals, J.

AU - Bons, J.

AU - van Oostenbrugge, R.J.

AU - Knottnerus, I.L.

AU - van Dieijen-Visser, M.P.

AU - Bouwman, F.G.

AU - Mariman, E.C.

AU - Delanghe, J.R.

AU - Lodder, J.

AU - Wodzig, W.K.

PY - 2008/1/1

Y1 - 2008/1/1

N2 - Using Surface-Enhanced Laser Desorption / Ionization Time-of-Flight Mass Spectrometry (SELDI-TOF-MS), we aimed to detect differences in protein profile in serum samples of two lacunar stroke subtypes. SELDI-TOF-MS, followed by protein identification, was performed in samples of 8 first-ever lacunar stroke patients with MR imaging showing a single symptomatic lacunar lesion (type 1), and 8 with multiple additional "silent" lacunar lesions and extensive white matter lesions (type 2). A 16 kDa protein, identified as alpha-2-chain of haptoglobin (Hp), was found to be overrepresented in type 1 compared to type 2 (peak intensity 12.5 vs. 5.0; p=0.02). As a polymorphism with two alleles, Hp-1 and Hp 2, determines the presence of alpha-1 and/or alpha-2-chains in the Hp-molecule, Hp phenotypic analysis was performed. Hp 1 : Hp-2 allele frequency was 0.562 : 0.438 in type 1 and 0.812 : 0.188 in type 2 (population reference approximately 0.4 : 0.6). We conclude that the overrepresentation of the alpha-2-chain in lacunar stroke type 1 compared to type 2 relates to a higher Hp-2 allele frequency in the former. Yet, compared to population reference, the phenotype distribution in both patient groups deviates towards a high Hp-1 allele frequency. Our findings suggest a role for the Hp gene in the etiology of cerebral small vessel disease. Larger studies are now needed to explore this new candidate gene.

AB - Using Surface-Enhanced Laser Desorption / Ionization Time-of-Flight Mass Spectrometry (SELDI-TOF-MS), we aimed to detect differences in protein profile in serum samples of two lacunar stroke subtypes. SELDI-TOF-MS, followed by protein identification, was performed in samples of 8 first-ever lacunar stroke patients with MR imaging showing a single symptomatic lacunar lesion (type 1), and 8 with multiple additional "silent" lacunar lesions and extensive white matter lesions (type 2). A 16 kDa protein, identified as alpha-2-chain of haptoglobin (Hp), was found to be overrepresented in type 1 compared to type 2 (peak intensity 12.5 vs. 5.0; p=0.02). As a polymorphism with two alleles, Hp-1 and Hp 2, determines the presence of alpha-1 and/or alpha-2-chains in the Hp-molecule, Hp phenotypic analysis was performed. Hp 1 : Hp-2 allele frequency was 0.562 : 0.438 in type 1 and 0.812 : 0.188 in type 2 (population reference approximately 0.4 : 0.6). We conclude that the overrepresentation of the alpha-2-chain in lacunar stroke type 1 compared to type 2 relates to a higher Hp-2 allele frequency in the former. Yet, compared to population reference, the phenotype distribution in both patient groups deviates towards a high Hp-1 allele frequency. Our findings suggest a role for the Hp gene in the etiology of cerebral small vessel disease. Larger studies are now needed to explore this new candidate gene.

U2 - 10.2174/156720208784310187

DO - 10.2174/156720208784310187

M3 - Article

VL - 5

SP - 93

EP - 98

JO - Current Neurovascular Research

JF - Current Neurovascular Research

SN - 1567-2026

IS - 2

ER -