A quantitative method to analyse F-actin distribution in cells

Jip Zonderland; Paul Wieringa; Lorenzo Moroni

doi:10.1016/j.mex.2019.10.018

A quantitative method to analyse F-actin distribution in cells

Jip Zonderland^*, Paul Wieringa, Lorenzo Moroni

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Changes in actin structure and distribution are involved in many cellular processes, such as differentiation, proliferation and migration. Differences in cell shape and size make the analysis of actin distribution difficult. Here, we have developed a Fiji macro that analyzes the distribution of actin within the cell, regardless of cell size or shape. The staining intensity is measured along an automatically drawn line over the cell. The intensity data is divided in equal bins, making the analysis insensitive to changes in cell size or shape. We have also created an R script that further processes the acquired data. Together, final data can be acquired within minutes from a set of images, with freely available software. We demonstrate our method with F-actin staining of cytochalasin D treated cells. The advantages of our methods are:

The analysis is not influenced by cell shape or size

All steps in the analysis are shown, and can therefore easily be verified for each image

Original language	English
Pages (from-to)	2562-2569
Number of pages	8
Journal	MethodsX
Volume	6
DOIs	https://doi.org/10.1016/j.mex.2019.10.018
Publication status	Published - 2019

Keywords

Fiji macro
Cytoskeleton distribution
Automated image quantification
Staining distribution
Mesenchymal stromal cells
Cytochalasin D
OSTEOBLASTS

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10.1016/j.mex.2019.10.018Licence: CC BY-NC-ND

1 Erratum / corrigendum / retractions

Corrigendum to “A quantitative method to analyse F-actin distribution in cells,” [MethodsX 6 (2019) 2562-2569]
Zonderland, J., Wieringa, P. & Moroni, L., 1 Jan 2023, In: MethodsX. 10, 1 p., 102139.
Research output: Contribution to journal › Erratum / corrigendum / retractions › Academic

Open Access

Cite this

@article{1a3f399e01814ef299db8123506f1c43,

title = "A quantitative method to analyse F-actin distribution in cells",

abstract = "Changes in actin structure and distribution are involved in many cellular processes, such as differentiation, proliferation and migration. Differences in cell shape and size make the analysis of actin distribution difficult. Here, we have developed a Fiji macro that analyzes the distribution of actin within the cell, regardless of cell size or shape. The staining intensity is measured along an automatically drawn line over the cell. The intensity data is divided in equal bins, making the analysis insensitive to changes in cell size or shape. We have also created an R script that further processes the acquired data. Together, final data can be acquired within minutes from a set of images, with freely available software. We demonstrate our method with F-actin staining of cytochalasin D treated cells. The advantages of our methods are:The analysis is not influenced by cell shape or sizeAll steps in the analysis are shown, and can therefore easily be verified for each imageAll software required for the analysis is freely available (C) 2019 The Authors. Published by Elsevier B.V.",

keywords = "Fiji macro, Cytoskeleton distribution, Automated image quantification, Staining distribution, Mesenchymal stromal cells, Cytochalasin D, OSTEOBLASTS",

author = "Jip Zonderland and Paul Wieringa and Lorenzo Moroni",

note = "Funding Information: We are grateful to the European Research Council starting grant “Cell Hybridge” for financial support under the Horizon2020 framework program (Grant # 637308 ). Appendix A Publisher Copyright: {\textcopyright} 2019 The Authors",

year = "2019",

doi = "10.1016/j.mex.2019.10.018",

language = "English",

volume = "6",

pages = "2562--2569",

journal = "MethodsX",

publisher = "Elsevier",

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TY - JOUR

T1 - A quantitative method to analyse F-actin distribution in cells

AU - Zonderland, Jip

AU - Wieringa, Paul

AU - Moroni, Lorenzo

N1 - Funding Information: We are grateful to the European Research Council starting grant “Cell Hybridge” for financial support under the Horizon2020 framework program (Grant # 637308 ). Appendix A Publisher Copyright: © 2019 The Authors

PY - 2019

Y1 - 2019

N2 - Changes in actin structure and distribution are involved in many cellular processes, such as differentiation, proliferation and migration. Differences in cell shape and size make the analysis of actin distribution difficult. Here, we have developed a Fiji macro that analyzes the distribution of actin within the cell, regardless of cell size or shape. The staining intensity is measured along an automatically drawn line over the cell. The intensity data is divided in equal bins, making the analysis insensitive to changes in cell size or shape. We have also created an R script that further processes the acquired data. Together, final data can be acquired within minutes from a set of images, with freely available software. We demonstrate our method with F-actin staining of cytochalasin D treated cells. The advantages of our methods are:The analysis is not influenced by cell shape or sizeAll steps in the analysis are shown, and can therefore easily be verified for each imageAll software required for the analysis is freely available (C) 2019 The Authors. Published by Elsevier B.V.

AB - Changes in actin structure and distribution are involved in many cellular processes, such as differentiation, proliferation and migration. Differences in cell shape and size make the analysis of actin distribution difficult. Here, we have developed a Fiji macro that analyzes the distribution of actin within the cell, regardless of cell size or shape. The staining intensity is measured along an automatically drawn line over the cell. The intensity data is divided in equal bins, making the analysis insensitive to changes in cell size or shape. We have also created an R script that further processes the acquired data. Together, final data can be acquired within minutes from a set of images, with freely available software. We demonstrate our method with F-actin staining of cytochalasin D treated cells. The advantages of our methods are:The analysis is not influenced by cell shape or sizeAll steps in the analysis are shown, and can therefore easily be verified for each imageAll software required for the analysis is freely available (C) 2019 The Authors. Published by Elsevier B.V.

KW - Fiji macro

KW - Cytoskeleton distribution

KW - Automated image quantification

KW - Staining distribution

KW - Mesenchymal stromal cells

KW - Cytochalasin D

KW - OSTEOBLASTS

U2 - 10.1016/j.mex.2019.10.018

DO - 10.1016/j.mex.2019.10.018

M3 - Article

C2 - 31763187

VL - 6

SP - 2562

EP - 2569

JO - MethodsX

JF - MethodsX

ER -

A quantitative method to analyse F-actin distribution in cells

Abstract

Keywords

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Research output

Corrigendum to “A quantitative method to analyse F-actin distribution in cells,” [MethodsX 6 (2019) 2562-2569]

Cite this