A pilot study assessing the feasibility of a short period of normothermic preservation in an experimental model of non heart beating donor kidneys.

S. A. Hosgood, A. D. Barlow, P. J. Yates, M.G. Snoeijs, L.W.E. van Heurn, M.L. Nicholson

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

BACKGROUND: Restoring metabolism to an organ after hypothermic storage and before transplantation could reverse some of the detrimental effects of ischemic injury. This may be particularly beneficial for kidneys from non-heart-beating (NHBD) donors that sustain significant periods of warm and cold ischemic injury. This pilot study assessed the feasibility of a short period of normothermic preservation (NP) in a porcine autotransplant model. METHODS: Kidneys were subjected to 30 min of warm ischemia, then preserved by hypothermic machine perfusion (HMP) for 22 h or 20 h HMP followed by 2 h of NP using autologous blood. Kidneys were then re-implanted, a contralateral nephrectomy performed, and renal function measured over 10 d. RESULTS: Post-transplant, 4/6 animals survived in the NP group compared with 5/6 in the HMP group (P = 1.00). Creatinine levels fell below 250 mumol/L in all four of the surviving animals in the NP group compared with 2/5 in the HMP group (P = 0.608). There was no difference in levels of renal function (peak creatinine, HMP= 1736 +/- 866 versus NP =1553 +/- 516 mumol/L; P >/= 0.990). Levels of lipid peroxidation were significantly lower 60 min post-transplant in the NP group (NP = 477 +/- 118.0 versus HMP = 671 +/- 99.4 pg/mL; P = 0.026). CONCLUSION: A period of NP at the end of the renal preservation period in NHB kidneys is a feasible method of kidney preservation. NP could prove to be a useful technique to predetermine graft function and allow pre-transplant modification of organs.
Original languageEnglish
Pages (from-to)283-290
Number of pages8
JournalJournal of Surgical Research
Volume171
Issue number1
DOIs
Publication statusPublished - Nov 2011

Keywords

  • hypothermic
  • ischemia
  • kidney
  • non-heart-beating donor
  • normothermic
  • preservation
  • porcine
  • DELAYED GRAFT FUNCTION
  • RENAL-TRANSPLANTATION
  • MACHINE PERFUSION
  • REPERFUSION INJURY
  • COLD-STORAGE
  • ISCHEMIA
  • WARM
  • BIOMARKERS
  • SURVIVAL
  • SYSTEM

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