A novel multiplexed 11 locus HLA full gene amplification assay using next generation sequencing

Keyphrases

• Next-generation Sequencing 100%
• HLA Typing 100%
• Multiplexing 100%
• Gene Amplification 100%
• Amplification Assay 100%
• Exon 40%
• Rapid Progress 20%
• Amplicon 20%
• Sequence Method 20%
• Sanger Sequencing 20%
• Read Depth 20%
• Gene Sequencing 20%
• HLA-A 20%
• DQA1 20%
• HLA-DQB1 20%
• Sequencing Platforms 20%
• Multiplex PCR (mPCR) 20%
• HLA Class I 20%
• Hematopoietic Stem Cell Transplantation 20%
• Reference Panel 20%
• Complex Allele 20%
• DPB1 20%
• DPA1 20%
• DRB1 20%
• DRB3 20%
• Stop Codon 20%
• Allele Groups 20%
• PCR Assay 20%
• HLA-B 20%
• HLA Class II 20%
• HLA-C 20%
• Typing Technique 20%
• HLA Class I Molecules 20%
• Next-generation Sequencing Platforms 20%
• Typing Ambiguity 20%
• Ion Torrent Sequencing 20%
• Novel Assay 20%

Immunology and Microbiology

• Next Generation Sequencing 100%
• Gene Amplification 100%
• HLA Typing 100%
• Allele 60%
• Exon 40%
• Gene Sequence 20%
• Amplicon 20%
• Hematopoietic Stem Cell Transplantation 20%
• Stop Codon 20%
• Dideoxynucleotide Sequencing 20%

INIS

• genes 100%
• gene amplification 100%
• information 25%
• resolution 25%
• pcr 25%
• exons 25%
• specificity 12%
• sensitivity 12%
• ions 12%
• control 12%
• comparative evaluations 12%
• dna 12%
• stem cells 12%
• panels 12%
• genetics 12%
• depth 12%
• codons 12%