A novel multiparameter flow cytometric assay for inosine triphosphatase expression analysis in leukocytes

Wim H. M. Vroemen; Imke C. A. Munnix; Jaap A. Bakker; Jorgen Bierau; Mark Huts; Mathie P. G. Leers

doi:10.1002/cyto.a.22051

A novel multiparameter flow cytometric assay for inosine triphosphatase expression analysis in leukocytes

Wim H. M. Vroemen, Imke C. A. Munnix, Jaap A. Bakker, Jorgen Bierau, Mark Huts, Mathie P. G. Leers^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The aim of this study was to assess inosine triphosphate (ITPase) expression in the different leukocyte populations present in peripheral blood samples of a nonimmune compromised control group. For this purpose, a multiparameter flow cytometric assay was developed and performed to study ITPase expression in peripheral leukocyte subpopulations of healthy volunteers (n = 20). Qualitative ITPase expression was assessed by determining the percentage of ITPase-positive cells. Quantitative data were obtained by measuring the median fluorescent intensity (MFI). Subcellular localization of ITPase was analyzed using immunocytochemistry. Immunocytochemistry showed that ITPase is present in all leukocytes and localized intracellular. Based on this finding, a multiparameter flow cytometric assay was developed using a Fix & Perm strategy. Qualitative and quantitative ITPase expression remained stable (variation,

Original language	English
Pages (from-to)	672-678
Journal	Cytometry Part A
Volume	81A
Issue number	8
DOIs	https://doi.org/10.1002/cyto.a.22051
Publication status	Published - Aug 2012

Keywords

ITPase
flow cytometry
leukocyte subpopulations
thiopurines

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10.1002/cyto.a.22051

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title = "A novel multiparameter flow cytometric assay for inosine triphosphatase expression analysis in leukocytes",

abstract = "The aim of this study was to assess inosine triphosphate (ITPase) expression in the different leukocyte populations present in peripheral blood samples of a nonimmune compromised control group. For this purpose, a multiparameter flow cytometric assay was developed and performed to study ITPase expression in peripheral leukocyte subpopulations of healthy volunteers (n = 20). Qualitative ITPase expression was assessed by determining the percentage of ITPase-positive cells. Quantitative data were obtained by measuring the median fluorescent intensity (MFI). Subcellular localization of ITPase was analyzed using immunocytochemistry. Immunocytochemistry showed that ITPase is present in all leukocytes and localized intracellular. Based on this finding, a multiparameter flow cytometric assay was developed using a Fix & Perm strategy. Qualitative and quantitative ITPase expression remained stable (variation, ",

keywords = "ITPase, flow cytometry, leukocyte subpopulations, thiopurines",

author = "Vroemen, {Wim H. M.} and Munnix, {Imke C. A.} and Bakker, {Jaap A.} and Jorgen Bierau and Mark Huts and Leers, {Mathie P. G.}",

year = "2012",

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T1 - A novel multiparameter flow cytometric assay for inosine triphosphatase expression analysis in leukocytes

AU - Vroemen, Wim H. M.

AU - Munnix, Imke C. A.

AU - Bakker, Jaap A.

AU - Bierau, Jorgen

AU - Huts, Mark

AU - Leers, Mathie P. G.

PY - 2012/8

Y1 - 2012/8

N2 - The aim of this study was to assess inosine triphosphate (ITPase) expression in the different leukocyte populations present in peripheral blood samples of a nonimmune compromised control group. For this purpose, a multiparameter flow cytometric assay was developed and performed to study ITPase expression in peripheral leukocyte subpopulations of healthy volunteers (n = 20). Qualitative ITPase expression was assessed by determining the percentage of ITPase-positive cells. Quantitative data were obtained by measuring the median fluorescent intensity (MFI). Subcellular localization of ITPase was analyzed using immunocytochemistry. Immunocytochemistry showed that ITPase is present in all leukocytes and localized intracellular. Based on this finding, a multiparameter flow cytometric assay was developed using a Fix & Perm strategy. Qualitative and quantitative ITPase expression remained stable (variation,

AB - The aim of this study was to assess inosine triphosphate (ITPase) expression in the different leukocyte populations present in peripheral blood samples of a nonimmune compromised control group. For this purpose, a multiparameter flow cytometric assay was developed and performed to study ITPase expression in peripheral leukocyte subpopulations of healthy volunteers (n = 20). Qualitative ITPase expression was assessed by determining the percentage of ITPase-positive cells. Quantitative data were obtained by measuring the median fluorescent intensity (MFI). Subcellular localization of ITPase was analyzed using immunocytochemistry. Immunocytochemistry showed that ITPase is present in all leukocytes and localized intracellular. Based on this finding, a multiparameter flow cytometric assay was developed using a Fix & Perm strategy. Qualitative and quantitative ITPase expression remained stable (variation,

KW - ITPase

KW - flow cytometry

KW - leukocyte subpopulations

KW - thiopurines

U2 - 10.1002/cyto.a.22051

DO - 10.1002/cyto.a.22051

M3 - Article

C2 - 22499283

SN - 1552-4922

VL - 81A

SP - 672

EP - 678

JO - Cytometry Part A

JF - Cytometry Part A

IS - 8

ER -

A novel multiparameter flow cytometric assay for inosine triphosphatase expression analysis in leukocytes

Abstract

Keywords

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