A novel experimental approach for three-dimensional geometry assessment of calcified human stenotic arteries in vitro

Renate W. Boekhoven*, Richard G P Lopata, Marc R. van Sambeek, Frans N van de Vosse, Marcel C. M. Rutten

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


To improve diagnosis and understanding of the risk of rupture of atherosclerotic plaque, new strategies to realistically determine mechanical properties of atherosclerotic plaque need to be developed. In this study, an in vitro experimental method is proposed for accurate 3-D assessment of (diseased) vessel geometry using ultrasound. The method was applied to a vascular phantom, a healthy porcine carotid artery and human carotid endarterectomy specimens (n = 6). Vessel segments were pressure fixed and rotated in 10 ° steps. Longitudinal cross sections were imaged over 360 °. Findings were validated using micro-computed tomography (μCT). Results show good agreement between ultrasound and μCT-based geometries of the different segment types (ISI phantom = 0.94, ISI healthy = 0.79, ISI diseased = 0.75-0.80). The method does not suffer from acoustic shadowing effects present when imaging stenotic segments and allows future dynamic measurements to determine mechanical properties of atherosclerotic plaque in an in vitro setting.

Original languageEnglish
Pages (from-to)1875-86
Number of pages12
JournalUltrasound in Medicine and Biology
Issue number10
Publication statusPublished - Oct 2013


  • Algorithms
  • Animals
  • Calcinosis
  • Carotid Arteries
  • Carotid Stenosis
  • Humans
  • Image Enhancement
  • Image Interpretation, Computer-Assisted
  • Imaging, Three-Dimensional
  • In Vitro Techniques
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Swine
  • Ultrasonography
  • Evaluation Studies
  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Validation Studies

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