A new approach to assess the total antioxidant capacity using the TEAC assay

M.J.T.J. Arts; J.W. Dallinga; H.P. Voss; G.R.M.M. Haenen; A. Bast

doi:10.1016/j.foodchem.2004.02.008

A new approach to assess the total antioxidant capacity using the TEAC assay

M.J.T.J. Arts^*, J.W. Dallinga, H.P. Voss, G.R.M.M. Haenen, A. Bast

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The trolox, equivalent antioxidant capacity (TEAC) assay is a popular method for assessing the capacity of a compound to scavenge ABTS radicals (ABTS(.)). Under the conditions in which the assay is performed, the reaction between most antioxidants and ABTS(.) does not reach completion within the time span applied. This leads to an underestimation of the TEAC of these antioxidants. In the present study, incubations with different concentrations of ABTS(.) and a fixed concentration of antioxidant were performed. The decrease in ABTS(.) concentration in 6 min was plotted against the initial concentration of ABTS(.) and fitted by an exponential function. Extrapolation of the fit to an infinite excess of ABTS(.) gives the maximal concentration of ABTS(.) that can be scavenged by the antioxidant at the concentration employed. This can be used to determine the actual TEAC of antioxidants, i.e. the total antioxidant capacity.

Original language	English
Pages (from-to)	567-570
Journal	Food Chemistry
Volume	88
DOIs	https://doi.org/10.1016/j.foodchem.2004.02.008
Publication status	Published - 1 Jan 2004

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10.1016/j.foodchem.2004.02.008

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title = "A new approach to assess the total antioxidant capacity using the TEAC assay",

abstract = "The trolox, equivalent antioxidant capacity (TEAC) assay is a popular method for assessing the capacity of a compound to scavenge ABTS radicals (ABTS(.)). Under the conditions in which the assay is performed, the reaction between most antioxidants and ABTS(.) does not reach completion within the time span applied. This leads to an underestimation of the TEAC of these antioxidants. In the present study, incubations with different concentrations of ABTS(.) and a fixed concentration of antioxidant were performed. The decrease in ABTS(.) concentration in 6 min was plotted against the initial concentration of ABTS(.) and fitted by an exponential function. Extrapolation of the fit to an infinite excess of ABTS(.) gives the maximal concentration of ABTS(.) that can be scavenged by the antioxidant at the concentration employed. This can be used to determine the actual TEAC of antioxidants, i.e. the total antioxidant capacity. ",

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T1 - A new approach to assess the total antioxidant capacity using the TEAC assay

AU - Arts, M.J.T.J.

AU - Dallinga, J.W.

AU - Voss, H.P.

AU - Haenen, G.R.M.M.

AU - Bast, A.

PY - 2004/1/1

Y1 - 2004/1/1

N2 - The trolox, equivalent antioxidant capacity (TEAC) assay is a popular method for assessing the capacity of a compound to scavenge ABTS radicals (ABTS(.)). Under the conditions in which the assay is performed, the reaction between most antioxidants and ABTS(.) does not reach completion within the time span applied. This leads to an underestimation of the TEAC of these antioxidants. In the present study, incubations with different concentrations of ABTS(.) and a fixed concentration of antioxidant were performed. The decrease in ABTS(.) concentration in 6 min was plotted against the initial concentration of ABTS(.) and fitted by an exponential function. Extrapolation of the fit to an infinite excess of ABTS(.) gives the maximal concentration of ABTS(.) that can be scavenged by the antioxidant at the concentration employed. This can be used to determine the actual TEAC of antioxidants, i.e. the total antioxidant capacity.

AB - The trolox, equivalent antioxidant capacity (TEAC) assay is a popular method for assessing the capacity of a compound to scavenge ABTS radicals (ABTS(.)). Under the conditions in which the assay is performed, the reaction between most antioxidants and ABTS(.) does not reach completion within the time span applied. This leads to an underestimation of the TEAC of these antioxidants. In the present study, incubations with different concentrations of ABTS(.) and a fixed concentration of antioxidant were performed. The decrease in ABTS(.) concentration in 6 min was plotted against the initial concentration of ABTS(.) and fitted by an exponential function. Extrapolation of the fit to an infinite excess of ABTS(.) gives the maximal concentration of ABTS(.) that can be scavenged by the antioxidant at the concentration employed. This can be used to determine the actual TEAC of antioxidants, i.e. the total antioxidant capacity.

U2 - 10.1016/j.foodchem.2004.02.008

DO - 10.1016/j.foodchem.2004.02.008

M3 - Article

SN - 0308-8146

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JO - Food Chemistry

JF - Food Chemistry

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