A Multi-Omics Approach Reveals Novel Regulators of Plasma Factor V Levels: highlight on CLEC4M as a Clearance Receptor

Gaëlle Munsch; Adarsh Mohapatra; Astrid van Hylckama Vlieg; Marcus E Kleber; Angel Martinez-Perez; Ngoc-Quynh Le; Kristian Dalsbø Hindberg; Philip J Dusart; Marine Germain; Florian Thibord; Jean-François Deleuze; Graciela E Delgado; Louisa Goumidi; Pierre Suchon; Noémie Saut; Juan Carlos Souto; Lynn Butler; Jose Manuel Soria; John-Bjarne Hansen; Winfried März; Frits R Rosendaal; Elisabetta Castoldi; Franck Peiretti; Maria Sabater-Lleal; David-Alexandre Tregouet; Pierre-Emmanuel Morange

doi:10.1182/blood.2024027006

A Multi-Omics Approach Reveals Novel Regulators of Plasma Factor V Levels: highlight on CLEC4M as a Clearance Receptor

Gaëlle Munsch
, Adarsh Mohapatra
, Astrid van Hylckama Vlieg
, Marcus E Kleber
, Angel Martinez-Perez
, Ngoc-Quynh Le
, Kristian Dalsbø Hindberg
, Philip J Dusart
, Marine Germain
, Florian Thibord
, Jean-François Deleuze
, Graciela E Delgado
, Louisa Goumidi
, Pierre Suchon
, Noémie Saut
, Juan Carlos Souto
, Lynn Butler
, Jose Manuel Soria
, John-Bjarne Hansen
, Winfried März

Frits R Rosendaal, Elisabetta Castoldi, Franck Peiretti, Maria Sabater-Lleal, David-Alexandre Tregouet^*, Pierre-Emmanuel Morange^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Coagulation factor V (FV) is a key protein in maintaining the hemostatic balance, with abnormal plasma levels associated with both thrombotic and hemorrhagic conditions. We propose a comprehensive bioinformatic analysis integrating large scale proteogenomics and transcriptomic data from original and public datasets. We identify a biological fingerprint of 26 new proteins and loci involved in the regulation of plasma FV levels. Furthermore, the mRNA expression levels of 10 of these components show strong correlation in liver. In addition, we provide experimental evidence for the involvement of one of the newly identified players (CLEC4M) in the clearance of FV. This work opens new avenues for a better understanding of the physiological processes involved in thrombotic and bleeding disorders.

Original language	English
Pages (from-to)	628-637
Number of pages	10
Journal	Blood
Volume	146
Issue number	5
Early online date	13 May 2025
DOIs	https://doi.org/10.1182/blood.2024027006
Publication status	Published - 31 Jul 2025

Access to Document

10.1182/blood.2024027006

Cite this

Munsch, G., Mohapatra, A., van Hylckama Vlieg, A., Kleber, M. E., Martinez-Perez, A., Le, N.-Q., Hindberg, K. D., Dusart, P. J., Germain, M., Thibord, F., Deleuze, J.-F., Delgado, G. E., Goumidi, L., Suchon, P., Saut, N., Souto, J. C., Butler, L., Soria, J. M., Hansen, J.-B., ... Morange, P.-E. (2025). A Multi-Omics Approach Reveals Novel Regulators of Plasma Factor V Levels: highlight on CLEC4M as a Clearance Receptor. Blood, 146(5), 628-637. https://doi.org/10.1182/blood.2024027006

@article{a92206dfbf424a70ad237179e76d7117,

title = "A Multi-Omics Approach Reveals Novel Regulators of Plasma Factor V Levels: highlight on CLEC4M as a Clearance Receptor",

abstract = "Coagulation factor V (FV) is a key protein in maintaining the hemostatic balance, with abnormal plasma levels associated with both thrombotic and hemorrhagic conditions. We propose a comprehensive bioinformatic analysis integrating large scale proteogenomics and transcriptomic data from original and public datasets. We identify a biological fingerprint of 26 new proteins and loci involved in the regulation of plasma FV levels. Furthermore, the mRNA expression levels of 10 of these components show strong correlation in liver. In addition, we provide experimental evidence for the involvement of one of the newly identified players (CLEC4M) in the clearance of FV. This work opens new avenues for a better understanding of the physiological processes involved in thrombotic and bleeding disorders.",

author = "Ga{\"e}lle Munsch and Adarsh Mohapatra and \{van Hylckama Vlieg\}, Astrid and Kleber, \{Marcus E\} and Angel Martinez-Perez and Ngoc-Quynh Le and Hindberg, \{Kristian Dalsb{\o}\} and Dusart, \{Philip J\} and Marine Germain and Florian Thibord and Jean-Fran{\c c}ois Deleuze and Delgado, \{Graciela E\} and Louisa Goumidi and Pierre Suchon and No{\'e}mie Saut and Souto, \{Juan Carlos\} and Lynn Butler and Soria, \{Jose Manuel\} and John-Bjarne Hansen and Winfried M{\"a}rz and Rosendaal, \{Frits R\} and Elisabetta Castoldi and Franck Peiretti and Maria Sabater-Lleal and David-Alexandre Tregouet and Pierre-Emmanuel Morange",

year = "2025",

month = jul,

day = "31",

doi = "10.1182/blood.2024027006",

language = "English",

volume = "146",

pages = "628--637",

journal = "Blood",

issn = "0006-4971",

publisher = "Elsevier B.V.",

number = "5",

}

Munsch, G, Mohapatra, A, van Hylckama Vlieg, A, Kleber, ME, Martinez-Perez, A, Le, N-Q, Hindberg, KD, Dusart, PJ, Germain, M, Thibord, F, Deleuze, J-F, Delgado, GE, Goumidi, L, Suchon, P, Saut, N, Souto, JC, Butler, L, Soria, JM, Hansen, J-B, März, W, Rosendaal, FR, Castoldi, E, Peiretti, F, Sabater-Lleal, M, Tregouet, D-A & Morange, P-E 2025, 'A Multi-Omics Approach Reveals Novel Regulators of Plasma Factor V Levels: highlight on CLEC4M as a Clearance Receptor', Blood, vol. 146, no. 5, pp. 628-637. https://doi.org/10.1182/blood.2024027006

TY - JOUR

T1 - A Multi-Omics Approach Reveals Novel Regulators of Plasma Factor V Levels

T2 - highlight on CLEC4M as a Clearance Receptor

AU - Munsch, Gaëlle

AU - Mohapatra, Adarsh

AU - van Hylckama Vlieg, Astrid

AU - Kleber, Marcus E

AU - Martinez-Perez, Angel

AU - Le, Ngoc-Quynh

AU - Hindberg, Kristian Dalsbø

AU - Dusart, Philip J

AU - Germain, Marine

AU - Thibord, Florian

AU - Deleuze, Jean-François

AU - Delgado, Graciela E

AU - Goumidi, Louisa

AU - Suchon, Pierre

AU - Saut, Noémie

AU - Souto, Juan Carlos

AU - Butler, Lynn

AU - Soria, Jose Manuel

AU - Hansen, John-Bjarne

AU - März, Winfried

AU - Rosendaal, Frits R

AU - Castoldi, Elisabetta

AU - Peiretti, Franck

AU - Sabater-Lleal, Maria

AU - Tregouet, David-Alexandre

AU - Morange, Pierre-Emmanuel

PY - 2025/7/31

Y1 - 2025/7/31

N2 - Coagulation factor V (FV) is a key protein in maintaining the hemostatic balance, with abnormal plasma levels associated with both thrombotic and hemorrhagic conditions. We propose a comprehensive bioinformatic analysis integrating large scale proteogenomics and transcriptomic data from original and public datasets. We identify a biological fingerprint of 26 new proteins and loci involved in the regulation of plasma FV levels. Furthermore, the mRNA expression levels of 10 of these components show strong correlation in liver. In addition, we provide experimental evidence for the involvement of one of the newly identified players (CLEC4M) in the clearance of FV. This work opens new avenues for a better understanding of the physiological processes involved in thrombotic and bleeding disorders.

AB - Coagulation factor V (FV) is a key protein in maintaining the hemostatic balance, with abnormal plasma levels associated with both thrombotic and hemorrhagic conditions. We propose a comprehensive bioinformatic analysis integrating large scale proteogenomics and transcriptomic data from original and public datasets. We identify a biological fingerprint of 26 new proteins and loci involved in the regulation of plasma FV levels. Furthermore, the mRNA expression levels of 10 of these components show strong correlation in liver. In addition, we provide experimental evidence for the involvement of one of the newly identified players (CLEC4M) in the clearance of FV. This work opens new avenues for a better understanding of the physiological processes involved in thrombotic and bleeding disorders.

U2 - 10.1182/blood.2024027006

DO - 10.1182/blood.2024027006

M3 - Article

SN - 0006-4971

VL - 146

SP - 628

EP - 637

JO - Blood

JF - Blood

IS - 5

ER -

A Multi-Omics Approach Reveals Novel Regulators of Plasma Factor V Levels: highlight on CLEC4M as a Clearance Receptor

Abstract

Access to Document

Fingerprint

Cite this