A fungicidal piperazine-1-carboxamidine induces mitochondrial fission-dependent apoptosis in yeast

Anna Bink; Gilmer Govaert; Isabelle E. J. A. Francois; Klaartje Pellens; Lieven Meerpoel; Marcel Borgers; Geert Van Minnebruggen; Valerie Vroome; Bruno P. A. Cammue; Karin Thevissen

doi:10.1111/j.1567-1364.2010.00663.x

A fungicidal piperazine-1-carboxamidine induces mitochondrial fission-dependent apoptosis in yeast

Anna Bink, Gilmer Govaert, Isabelle E. J. A. Francois, Klaartje Pellens, Lieven Meerpoel, Marcel Borgers, Geert Van Minnebruggen, Valerie Vroome, Bruno P. A. Cammue^*, Karin Thevissen

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

To unravel the working mechanism of the fungicidal piperazine-1-carboxamidine derivative BAR0329, we found that its intracellular accumulation in Saccharomyces cerevisiae is dependent on functional lipid rafts. Moreover, BAR0329 induced caspase-dependent apoptosis in yeast, in which the mitochondrial fission machinery consisting of Fis1 (Whi2), Dnm1 and Mdv1 is involved. Our data are consistent with a prosurvival function of Fis1 (Whi2) and a proapoptotic function of Dnm1 and Mdv1 during BAR0329-induced yeast cell death.

Original language	English
Pages (from-to)	812-818
Journal	Fems Yeast Research
Volume	10
Issue number	7
DOIs	https://doi.org/10.1111/j.1567-1364.2010.00663.x
Publication status	Published - Nov 2010

Keywords

apoptosis
fungicidal
lipid raft
caspase
mitochondrial fission machinery
piperazine-1-carboxamidine

Access to Document

10.1111/j.1567-1364.2010.00663.x

Cite this

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title = "A fungicidal piperazine-1-carboxamidine induces mitochondrial fission-dependent apoptosis in yeast",

abstract = "To unravel the working mechanism of the fungicidal piperazine-1-carboxamidine derivative BAR0329, we found that its intracellular accumulation in Saccharomyces cerevisiae is dependent on functional lipid rafts. Moreover, BAR0329 induced caspase-dependent apoptosis in yeast, in which the mitochondrial fission machinery consisting of Fis1 (Whi2), Dnm1 and Mdv1 is involved. Our data are consistent with a prosurvival function of Fis1 (Whi2) and a proapoptotic function of Dnm1 and Mdv1 during BAR0329-induced yeast cell death.",

keywords = "apoptosis, fungicidal, lipid raft, caspase, mitochondrial fission machinery, piperazine-1-carboxamidine",

author = "Anna Bink and Gilmer Govaert and Francois, {Isabelle E. J. A.} and Klaartje Pellens and Lieven Meerpoel and Marcel Borgers and {Van Minnebruggen}, Geert and Valerie Vroome and Cammue, {Bruno P. A.} and Karin Thevissen",

year = "2010",

month = nov,

doi = "10.1111/j.1567-1364.2010.00663.x",

language = "English",

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journal = "Fems Yeast Research",

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T1 - A fungicidal piperazine-1-carboxamidine induces mitochondrial fission-dependent apoptosis in yeast

AU - Bink, Anna

AU - Govaert, Gilmer

AU - Francois, Isabelle E. J. A.

AU - Pellens, Klaartje

AU - Meerpoel, Lieven

AU - Borgers, Marcel

AU - Van Minnebruggen, Geert

AU - Vroome, Valerie

AU - Cammue, Bruno P. A.

AU - Thevissen, Karin

PY - 2010/11

Y1 - 2010/11

N2 - To unravel the working mechanism of the fungicidal piperazine-1-carboxamidine derivative BAR0329, we found that its intracellular accumulation in Saccharomyces cerevisiae is dependent on functional lipid rafts. Moreover, BAR0329 induced caspase-dependent apoptosis in yeast, in which the mitochondrial fission machinery consisting of Fis1 (Whi2), Dnm1 and Mdv1 is involved. Our data are consistent with a prosurvival function of Fis1 (Whi2) and a proapoptotic function of Dnm1 and Mdv1 during BAR0329-induced yeast cell death.

AB - To unravel the working mechanism of the fungicidal piperazine-1-carboxamidine derivative BAR0329, we found that its intracellular accumulation in Saccharomyces cerevisiae is dependent on functional lipid rafts. Moreover, BAR0329 induced caspase-dependent apoptosis in yeast, in which the mitochondrial fission machinery consisting of Fis1 (Whi2), Dnm1 and Mdv1 is involved. Our data are consistent with a prosurvival function of Fis1 (Whi2) and a proapoptotic function of Dnm1 and Mdv1 during BAR0329-induced yeast cell death.

KW - apoptosis

KW - fungicidal

KW - lipid raft

KW - caspase

KW - mitochondrial fission machinery

KW - piperazine-1-carboxamidine

U2 - 10.1111/j.1567-1364.2010.00663.x

DO - 10.1111/j.1567-1364.2010.00663.x

M3 - Article

C2 - 20726898

SN - 1567-1356

VL - 10

SP - 812

EP - 818

JO - Fems Yeast Research

JF - Fems Yeast Research

IS - 7

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