A fungicidal piperazine-1-carboxamidine induces mitochondrial fission-dependent apoptosis in yeast

Anna Bink, Gilmer Govaert, Isabelle E. J. A. Francois, Klaartje Pellens, Lieven Meerpoel, Marcel Borgers, Geert Van Minnebruggen, Valerie Vroome, Bruno P. A. Cammue*, Karin Thevissen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Web of Science)

Abstract

To unravel the working mechanism of the fungicidal piperazine-1-carboxamidine derivative BAR0329, we found that its intracellular accumulation in Saccharomyces cerevisiae is dependent on functional lipid rafts. Moreover, BAR0329 induced caspase-dependent apoptosis in yeast, in which the mitochondrial fission machinery consisting of Fis1 (Whi2), Dnm1 and Mdv1 is involved. Our data are consistent with a prosurvival function of Fis1 (Whi2) and a proapoptotic function of Dnm1 and Mdv1 during BAR0329-induced yeast cell death.
Original languageEnglish
Pages (from-to)812-818
JournalFems Yeast Research
Volume10
Issue number7
DOIs
Publication statusPublished - Nov 2010

Keywords

  • apoptosis
  • fungicidal
  • lipid raft
  • caspase
  • mitochondrial fission machinery
  • piperazine-1-carboxamidine

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