A Facile Approach for Doxorubicine Delivery in Cancer Cells by Responsive and Fluorescent Core/Shell Quantum Dots

Enaam Jamal Al Dine; Sophie Marchal; Raphael Schneider; Batoul Hamie; Jaafar Ghanbaja; Thibault Roques-Carmes; Tayssir Hamieh; Joumana Toufaily; Eric Gaifet; Halima Alem

doi:10.1021/acs.bioconjchem.8b00253

A Facile Approach for Doxorubicine Delivery in Cancer Cells by Responsive and Fluorescent Core/Shell Quantum Dots

Enaam Jamal Al Dine, Sophie Marchal, Raphael Schneider, Batoul Hamie, Jaafar Ghanbaja, Thibault Roques-Carmes, Tayssir Hamieh, Joumana Toufaily, Eric Gaifet, Halima Alem^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Biocompatible thermoresponsive copolymers based on 2-(2-methoxyethoxy) ethyl methacrylate (MEO ₂MA) and oligo (ethylene glycol) methacrylate (OEGMA) were grown from the surface of ZnO quantum dots (QDs) by surface initiated atom transfer radical polymerization with activators regenerated by electron transfer (SI-ARGET ATRP) in order to design smart and fluorescent core/shell nanosystems to be used toward cancer cells. Tunable lower critical solution temperature (LCST) values were obtained and studied in water and in culture medium. The complete efficiency of the process was demonstrated by the combination of spectroscopic and microscopic studies. The colloidal behavior of the ZnO/copolymer core/shell QDs in water and in physiological media with temperature was assessed. Finally, the cytotoxicity toward human colon cancer HT29 cells of the core/shell QDs was tested. The results showed that the polymer-capped QDs exhibited almost no toxicity at concentrations up to 12.5 μg.mL ^-1, while when loaded with doxorubicin hydrochloride (DOX), a higher cytotoxicity and a decreased HT29 cancer cell viability in a short time were observed.

Original language	English
Pages (from-to)	2248-2256
Number of pages	9
Journal	Bioconjugate Chemistry
Volume	29
Issue number	7
DOIs	https://doi.org/10.1021/acs.bioconjchem.8b00253
Publication status	Published - 18 Jul 2018
Externally published	Yes

Keywords

CORE-SHELL NANOPARTICLES
TARGETED DRUG-DELIVERY
HIGH NIR ABSORBENCY
INTEGRAL-EQUATIONS
POLYMERS
RELEASE
THERAPY

Access to Document

10.1021/acs.bioconjchem.8b00253

Cite this

@article{8e23cc7020154258b43d78e4e410a9ca,

title = "A Facile Approach for Doxorubicine Delivery in Cancer Cells by Responsive and Fluorescent Core/Shell Quantum Dots",

abstract = "Biocompatible thermoresponsive copolymers based on 2-(2-methoxyethoxy) ethyl methacrylate (MEO 2MA) and oligo (ethylene glycol) methacrylate (OEGMA) were grown from the surface of ZnO quantum dots (QDs) by surface initiated atom transfer radical polymerization with activators regenerated by electron transfer (SI-ARGET ATRP) in order to design smart and fluorescent core/shell nanosystems to be used toward cancer cells. Tunable lower critical solution temperature (LCST) values were obtained and studied in water and in culture medium. The complete efficiency of the process was demonstrated by the combination of spectroscopic and microscopic studies. The colloidal behavior of the ZnO/copolymer core/shell QDs in water and in physiological media with temperature was assessed. Finally, the cytotoxicity toward human colon cancer HT29 cells of the core/shell QDs was tested. The results showed that the polymer-capped QDs exhibited almost no toxicity at concentrations up to 12.5 μg.mL -1, while when loaded with doxorubicin hydrochloride (DOX), a higher cytotoxicity and a decreased HT29 cancer cell viability in a short time were observed.",

keywords = "CORE-SHELL NANOPARTICLES, TARGETED DRUG-DELIVERY, HIGH NIR ABSORBENCY, INTEGRAL-EQUATIONS, POLYMERS, RELEASE, THERAPY",

author = "\{Al Dine\}, \{Enaam Jamal\} and Sophie Marchal and Raphael Schneider and Batoul Hamie and Jaafar Ghanbaja and Thibault Roques-Carmes and Tayssir Hamieh and Joumana Toufaily and Eric Gaifet and Halima Alem",

year = "2018",

month = jul,

day = "18",

doi = "10.1021/acs.bioconjchem.8b00253",

language = "English",

volume = "29",

pages = "2248--2256",

journal = "Bioconjugate Chemistry",

issn = "1043-1802",

publisher = "American Chemical Society",

number = "7",

}

TY - JOUR

T1 - A Facile Approach for Doxorubicine Delivery in Cancer Cells by Responsive and Fluorescent Core/Shell Quantum Dots

AU - Al Dine, Enaam Jamal

AU - Marchal, Sophie

AU - Schneider, Raphael

AU - Hamie, Batoul

AU - Ghanbaja, Jaafar

AU - Roques-Carmes, Thibault

AU - Hamieh, Tayssir

AU - Toufaily, Joumana

AU - Gaifet, Eric

AU - Alem, Halima

PY - 2018/7/18

Y1 - 2018/7/18

N2 - Biocompatible thermoresponsive copolymers based on 2-(2-methoxyethoxy) ethyl methacrylate (MEO 2MA) and oligo (ethylene glycol) methacrylate (OEGMA) were grown from the surface of ZnO quantum dots (QDs) by surface initiated atom transfer radical polymerization with activators regenerated by electron transfer (SI-ARGET ATRP) in order to design smart and fluorescent core/shell nanosystems to be used toward cancer cells. Tunable lower critical solution temperature (LCST) values were obtained and studied in water and in culture medium. The complete efficiency of the process was demonstrated by the combination of spectroscopic and microscopic studies. The colloidal behavior of the ZnO/copolymer core/shell QDs in water and in physiological media with temperature was assessed. Finally, the cytotoxicity toward human colon cancer HT29 cells of the core/shell QDs was tested. The results showed that the polymer-capped QDs exhibited almost no toxicity at concentrations up to 12.5 μg.mL -1, while when loaded with doxorubicin hydrochloride (DOX), a higher cytotoxicity and a decreased HT29 cancer cell viability in a short time were observed.

AB - Biocompatible thermoresponsive copolymers based on 2-(2-methoxyethoxy) ethyl methacrylate (MEO 2MA) and oligo (ethylene glycol) methacrylate (OEGMA) were grown from the surface of ZnO quantum dots (QDs) by surface initiated atom transfer radical polymerization with activators regenerated by electron transfer (SI-ARGET ATRP) in order to design smart and fluorescent core/shell nanosystems to be used toward cancer cells. Tunable lower critical solution temperature (LCST) values were obtained and studied in water and in culture medium. The complete efficiency of the process was demonstrated by the combination of spectroscopic and microscopic studies. The colloidal behavior of the ZnO/copolymer core/shell QDs in water and in physiological media with temperature was assessed. Finally, the cytotoxicity toward human colon cancer HT29 cells of the core/shell QDs was tested. The results showed that the polymer-capped QDs exhibited almost no toxicity at concentrations up to 12.5 μg.mL -1, while when loaded with doxorubicin hydrochloride (DOX), a higher cytotoxicity and a decreased HT29 cancer cell viability in a short time were observed.

KW - CORE-SHELL NANOPARTICLES

KW - TARGETED DRUG-DELIVERY

KW - HIGH NIR ABSORBENCY

KW - INTEGRAL-EQUATIONS

KW - POLYMERS

KW - RELEASE

KW - THERAPY

U2 - 10.1021/acs.bioconjchem.8b00253

DO - 10.1021/acs.bioconjchem.8b00253

M3 - Article

SN - 1043-1802

VL - 29

SP - 2248

EP - 2256

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

IS - 7

ER -

A Facile Approach for Doxorubicine Delivery in Cancer Cells by Responsive and Fluorescent Core/Shell Quantum Dots

Abstract

Keywords

Access to Document

Fingerprint

Cite this