A dual-labeled Annexin A5 is not suited for SPECT imaging of brain cell death in experimental murine stroke

Marietta Zille; Denise Harhausenl; Marijke De Saint-Hubert; Roger Michel; Chris P. Reutelingsperger; Ulrich Dirnagl; Andreas Wunder

doi:10.1038/jcbfm.2014.115

A dual-labeled Annexin A5 is not suited for SPECT imaging of brain cell death in experimental murine stroke

Marietta Zille, Denise Harhausenl, Marijke De Saint-Hubert, Roger Michel, Chris P. Reutelingsperger, Ulrich Dirnagl^*, Andreas Wunder

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We previously showed that fluorescently labeled Annexin A5 (AnxA5); which binds specifically to phosphatidylserine on dead/dying cells, can be used in experimental stroke for monitoring cell death with optical imaging. However, optical imaging is not suitable for brain imaging in patients. Here we investigated whether a dual-labeled AnxA5 (technetium and fluorescence label) can be used for single-photon emission computed tomography (SPECT) of cell death in the same model. We detected dual-labeled AnxA5 in ethanol-induced cell death in the femur muscle, but not after middle cerebral artery occlusion (MCAO) at any time point, either with SPECT or with ex vivo autoradiography or fluorescence Microscopy. Dual-labeled AnxA5 appears to be unsuited for visualizing death of brain cells in this MCAO model.

Original language	English
Pages (from-to)	1568-1570
Journal	Journal of Cerebral Blood Flow and Metabolism
Volume	34
Issue number	9
DOIs	https://doi.org/10.1038/jcbfm.2014.115
Publication status	Published - Sept 2014

Keywords

apoptosis
cerebral ischemia
mouse
phosphatidylserine
single-photon emission computed tomography

Access to Document

10.1038/jcbfm.2014.115

Cite this

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title = "A dual-labeled Annexin A5 is not suited for SPECT imaging of brain cell death in experimental murine stroke",

abstract = "We previously showed that fluorescently labeled Annexin A5 (AnxA5); which binds specifically to phosphatidylserine on dead/dying cells, can be used in experimental stroke for monitoring cell death with optical imaging. However, optical imaging is not suitable for brain imaging in patients. Here we investigated whether a dual-labeled AnxA5 (technetium and fluorescence label) can be used for single-photon emission computed tomography (SPECT) of cell death in the same model. We detected dual-labeled AnxA5 in ethanol-induced cell death in the femur muscle, but not after middle cerebral artery occlusion (MCAO) at any time point, either with SPECT or with ex vivo autoradiography or fluorescence Microscopy. Dual-labeled AnxA5 appears to be unsuited for visualizing death of brain cells in this MCAO model.",

keywords = "apoptosis, cerebral ischemia, mouse, phosphatidylserine, single-photon emission computed tomography",

author = "Marietta Zille and Denise Harhausenl and {De Saint-Hubert}, Marijke and Roger Michel and Reutelingsperger, {Chris P.} and Ulrich Dirnagl and Andreas Wunder",

year = "2014",

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T1 - A dual-labeled Annexin A5 is not suited for SPECT imaging of brain cell death in experimental murine stroke

AU - Zille, Marietta

AU - Harhausenl, Denise

AU - De Saint-Hubert, Marijke

AU - Michel, Roger

AU - Reutelingsperger, Chris P.

AU - Dirnagl, Ulrich

AU - Wunder, Andreas

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AB - We previously showed that fluorescently labeled Annexin A5 (AnxA5); which binds specifically to phosphatidylserine on dead/dying cells, can be used in experimental stroke for monitoring cell death with optical imaging. However, optical imaging is not suitable for brain imaging in patients. Here we investigated whether a dual-labeled AnxA5 (technetium and fluorescence label) can be used for single-photon emission computed tomography (SPECT) of cell death in the same model. We detected dual-labeled AnxA5 in ethanol-induced cell death in the femur muscle, but not after middle cerebral artery occlusion (MCAO) at any time point, either with SPECT or with ex vivo autoradiography or fluorescence Microscopy. Dual-labeled AnxA5 appears to be unsuited for visualizing death of brain cells in this MCAO model.

KW - apoptosis

KW - cerebral ischemia

KW - mouse

KW - phosphatidylserine

KW - single-photon emission computed tomography

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