A dual-labeled Annexin A5 is not suited for SPECT imaging of brain cell death in experimental murine stroke

Marietta Zille, Denise Harhausenl, Marijke De Saint-Hubert, Roger Michel, Chris P. Reutelingsperger, Ulrich Dirnagl*, Andreas Wunder

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Web of Science)

Abstract

We previously showed that fluorescently labeled Annexin A5 (AnxA5); which binds specifically to phosphatidylserine on dead/dying cells, can be used in experimental stroke for monitoring cell death with optical imaging. However, optical imaging is not suitable for brain imaging in patients. Here we investigated whether a dual-labeled AnxA5 (technetium and fluorescence label) can be used for single-photon emission computed tomography (SPECT) of cell death in the same model. We detected dual-labeled AnxA5 in ethanol-induced cell death in the femur muscle, but not after middle cerebral artery occlusion (MCAO) at any time point, either with SPECT or with ex vivo autoradiography or fluorescence Microscopy. Dual-labeled AnxA5 appears to be unsuited for visualizing death of brain cells in this MCAO model.
Original languageEnglish
Pages (from-to)1568-1570
JournalJournal of Cerebral Blood Flow and Metabolism
Volume34
Issue number9
DOIs
Publication statusPublished - Sep 2014

Keywords

  • apoptosis
  • cerebral ischemia
  • mouse
  • phosphatidylserine
  • single-photon emission computed tomography

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