A combinatorial approach towards the design of nanofibrous scaffolds for chondrogenesis

Maqsood Ahmed; Tiago Andre da Silva Ramos; Febriyani Damanik; Bach Quang Le; Paul Wieringa; Martin Bennink; Clemens van Blitterswijk; Jan de Boer; Lorenzo Moroni

doi:10.1038/srep14804

A combinatorial approach towards the design of nanofibrous scaffolds for chondrogenesis

Maqsood Ahmed, Tiago Andre da Silva Ramos, Febriyani Damanik, Bach Quang Le, Paul Wieringa, Martin Bennink, Clemens van Blitterswijk, Jan de Boer, Lorenzo Moroni^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The extracellular matrix (ECM) is a three-dimensional (3D) structure composed of proteinaceous fibres that provide physical and biological cues to direct cell behaviour. Here, we build a library of hybrid collagen-polymer fibrous scaffolds with nanoscale dimensions and screen them for their ability to grow chondrocytes for cartilage repair. Poly(lactic acid) and poly (lactic-co-glycolic acid) at two different monomer ratios (85:15 and 50:50) were incrementally blended with collagen. Physical properties (wettability and stiffness) of the scaffolds were characterized and related to biological performance (proliferation, ECM production, and gene expression) and structure-function relationships were developed. We found that soft scaffolds with an intermediate wettability composed of the highly biodegradable PLGA50:50 and collagen, in two ratios (40:60 and 60:40), were optimal for chondrogenic differentiation of ATDC5 cells as determined by increased ECM production and enhanced cartilage specific gene expression. Long-term cultures indicated a stable phenotype with minimal de-differentiation or hypertrophy. The combinatorial methodology applied herein is a promising approach for the design and development of scaffolds for regenerative medicine.

Original language	English
Article number	14804
Journal	Scientific Reports
Volume	5
DOIs	https://doi.org/10.1038/srep14804
Publication status	Published - 7 Oct 2015

Access to Document

10.1038/srep14804Licence: CC BY

Cite this

@article{66da4a9cdce84ec199a161ab00e18856,

title = "A combinatorial approach towards the design of nanofibrous scaffolds for chondrogenesis",

abstract = "The extracellular matrix (ECM) is a three-dimensional (3D) structure composed of proteinaceous fibres that provide physical and biological cues to direct cell behaviour. Here, we build a library of hybrid collagen-polymer fibrous scaffolds with nanoscale dimensions and screen them for their ability to grow chondrocytes for cartilage repair. Poly(lactic acid) and poly (lactic-co-glycolic acid) at two different monomer ratios (85:15 and 50:50) were incrementally blended with collagen. Physical properties (wettability and stiffness) of the scaffolds were characterized and related to biological performance (proliferation, ECM production, and gene expression) and structure-function relationships were developed. We found that soft scaffolds with an intermediate wettability composed of the highly biodegradable PLGA50:50 and collagen, in two ratios (40:60 and 60:40), were optimal for chondrogenic differentiation of ATDC5 cells as determined by increased ECM production and enhanced cartilage specific gene expression. Long-term cultures indicated a stable phenotype with minimal de-differentiation or hypertrophy. The combinatorial methodology applied herein is a promising approach for the design and development of scaffolds for regenerative medicine.",

author = "Maqsood Ahmed and {da Silva Ramos}, {Tiago Andre} and Febriyani Damanik and {Bach Quang Le} and Paul Wieringa and Martin Bennink and {van Blitterswijk}, Clemens and {de Boer}, Jan and Lorenzo Moroni",

year = "2015",

month = oct,

day = "7",

doi = "10.1038/srep14804",

language = "English",

volume = "5",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - A combinatorial approach towards the design of nanofibrous scaffolds for chondrogenesis

AU - Ahmed, Maqsood

AU - da Silva Ramos, Tiago Andre

AU - Damanik, Febriyani

AU - Bach Quang Le,

AU - Wieringa, Paul

AU - Bennink, Martin

AU - van Blitterswijk, Clemens

AU - de Boer, Jan

AU - Moroni, Lorenzo

PY - 2015/10/7

Y1 - 2015/10/7

N2 - The extracellular matrix (ECM) is a three-dimensional (3D) structure composed of proteinaceous fibres that provide physical and biological cues to direct cell behaviour. Here, we build a library of hybrid collagen-polymer fibrous scaffolds with nanoscale dimensions and screen them for their ability to grow chondrocytes for cartilage repair. Poly(lactic acid) and poly (lactic-co-glycolic acid) at two different monomer ratios (85:15 and 50:50) were incrementally blended with collagen. Physical properties (wettability and stiffness) of the scaffolds were characterized and related to biological performance (proliferation, ECM production, and gene expression) and structure-function relationships were developed. We found that soft scaffolds with an intermediate wettability composed of the highly biodegradable PLGA50:50 and collagen, in two ratios (40:60 and 60:40), were optimal for chondrogenic differentiation of ATDC5 cells as determined by increased ECM production and enhanced cartilage specific gene expression. Long-term cultures indicated a stable phenotype with minimal de-differentiation or hypertrophy. The combinatorial methodology applied herein is a promising approach for the design and development of scaffolds for regenerative medicine.

AB - The extracellular matrix (ECM) is a three-dimensional (3D) structure composed of proteinaceous fibres that provide physical and biological cues to direct cell behaviour. Here, we build a library of hybrid collagen-polymer fibrous scaffolds with nanoscale dimensions and screen them for their ability to grow chondrocytes for cartilage repair. Poly(lactic acid) and poly (lactic-co-glycolic acid) at two different monomer ratios (85:15 and 50:50) were incrementally blended with collagen. Physical properties (wettability and stiffness) of the scaffolds were characterized and related to biological performance (proliferation, ECM production, and gene expression) and structure-function relationships were developed. We found that soft scaffolds with an intermediate wettability composed of the highly biodegradable PLGA50:50 and collagen, in two ratios (40:60 and 60:40), were optimal for chondrogenic differentiation of ATDC5 cells as determined by increased ECM production and enhanced cartilage specific gene expression. Long-term cultures indicated a stable phenotype with minimal de-differentiation or hypertrophy. The combinatorial methodology applied herein is a promising approach for the design and development of scaffolds for regenerative medicine.

U2 - 10.1038/srep14804

DO - 10.1038/srep14804

M3 - Article

C2 - 26445026

SN - 2045-2322

VL - 5

JO - Scientific Reports

JF - Scientific Reports

M1 - 14804

ER -