A Citrus Fruit Extract High in Polyphenols Beneficially Modulates the Gut Microbiota of Healthy Human Volunteers in a Validated In Vitro Model of the Colon

M.M. Sost; S. Ahles; J. Verhoeven; S. Verbruggen; Y. Stevens; K. Venema

doi:10.3390/nu13113915

A Citrus Fruit Extract High in Polyphenols Beneficially Modulates the Gut Microbiota of Healthy Human Volunteers in a Validated In Vitro Model of the Colon

M.M. Sost, S. Ahles, J. Verhoeven, S. Verbruggen, Y. Stevens, K. Venema^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The effect of a Citrus Fruit Extract high in the polyphenols hesperidin and naringin (CFE) on modulation of the composition and activity of the gut microbiota was tested in a validated, dynamic in vitro model of the colon (TIM-2). CFE was provided at two doses (250 and 350 mg/day) for 3 days. CFE led to a dose-dependent increase in Roseburia, Eubacterium ramulus, and Bacteroides eggerthii. There was a shift in production of short-chain fatty acids, where acetate production increased on CFE, while butyrate decreased. In overweight and obesity, acetate has been shown to increase fat oxidation when produced in the distal gut, and stimulate secretion of appetite-suppressive neuropeptides. Thus, the data in the in vitro model point towards mechanisms underlying the effects of the polyphenols in CFE with respect to modulation of the gut microbiota, both in composition and activity. These results should be confirmed in a clinical trial.

Original language	English
Article number	3915
Number of pages	13
Journal	Nutrients
Volume	13
Issue number	11
DOIs	https://doi.org/10.3390/nu13113915
Publication status	Published - 1 Nov 2021

Keywords

Roseburia
Citrus Fruit Extract
gut microbiota
short-chain fatty acids (SCFA)
in vitro model
TIM-2
CHAIN FATTY-ACIDS
METABOLIC SYNDROME
FECAL MICROBIOTA
LARGE-INTESTINE
PROPIONIC-ACID
FERMENTATION
ACETATE
IMPACT
PHYSIOLOGY
INFUSIONS

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@article{9666e8083cf645dd80f9d17db5a8d82d,

title = "A Citrus Fruit Extract High in Polyphenols Beneficially Modulates the Gut Microbiota of Healthy Human Volunteers in a Validated In Vitro Model of the Colon",

abstract = "The effect of a Citrus Fruit Extract high in the polyphenols hesperidin and naringin (CFE) on modulation of the composition and activity of the gut microbiota was tested in a validated, dynamic in vitro model of the colon (TIM-2). CFE was provided at two doses (250 and 350 mg/day) for 3 days. CFE led to a dose-dependent increase in Roseburia, Eubacterium ramulus, and Bacteroides eggerthii. There was a shift in production of short-chain fatty acids, where acetate production increased on CFE, while butyrate decreased. In overweight and obesity, acetate has been shown to increase fat oxidation when produced in the distal gut, and stimulate secretion of appetite-suppressive neuropeptides. Thus, the data in the in vitro model point towards mechanisms underlying the effects of the polyphenols in CFE with respect to modulation of the gut microbiota, both in composition and activity. These results should be confirmed in a clinical trial.",

keywords = "Roseburia, Citrus Fruit Extract, gut microbiota, short-chain fatty acids (SCFA), in vitro model, TIM-2, CHAIN FATTY-ACIDS, METABOLIC SYNDROME, FECAL MICROBIOTA, LARGE-INTESTINE, PROPIONIC-ACID, FERMENTATION, ACETATE, IMPACT, PHYSIOLOGY, INFUSIONS",

author = "M.M. Sost and S. Ahles and J. Verhoeven and S. Verbruggen and Y. Stevens and K. Venema",

note = "Funding Information: Funding: This research was funded by BioActor and has been made possible with the support of the Dutch Province of Limburg with a Grant to HEFI, grant HEFI-1. The APC was funded by Maastricht University–campus Venlo. Funding Information: This research was funded by BioActor and has been made possible with the support of the Dutch Province of Limburg with a Grant to HEFI, grant HEFI-1. The APC was funded by Maastricht University?campus Venlo. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

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doi = "10.3390/nu13113915",

language = "English",

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AU - Sost, M.M.

AU - Ahles, S.

AU - Verhoeven, J.

AU - Verbruggen, S.

AU - Stevens, Y.

AU - Venema, K.

N1 - Funding Information: Funding: This research was funded by BioActor and has been made possible with the support of the Dutch Province of Limburg with a Grant to HEFI, grant HEFI-1. The APC was funded by Maastricht University–campus Venlo. Funding Information: This research was funded by BioActor and has been made possible with the support of the Dutch Province of Limburg with a Grant to HEFI, grant HEFI-1. The APC was funded by Maastricht University?campus Venlo. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/11/1

Y1 - 2021/11/1

N2 - The effect of a Citrus Fruit Extract high in the polyphenols hesperidin and naringin (CFE) on modulation of the composition and activity of the gut microbiota was tested in a validated, dynamic in vitro model of the colon (TIM-2). CFE was provided at two doses (250 and 350 mg/day) for 3 days. CFE led to a dose-dependent increase in Roseburia, Eubacterium ramulus, and Bacteroides eggerthii. There was a shift in production of short-chain fatty acids, where acetate production increased on CFE, while butyrate decreased. In overweight and obesity, acetate has been shown to increase fat oxidation when produced in the distal gut, and stimulate secretion of appetite-suppressive neuropeptides. Thus, the data in the in vitro model point towards mechanisms underlying the effects of the polyphenols in CFE with respect to modulation of the gut microbiota, both in composition and activity. These results should be confirmed in a clinical trial.

AB - The effect of a Citrus Fruit Extract high in the polyphenols hesperidin and naringin (CFE) on modulation of the composition and activity of the gut microbiota was tested in a validated, dynamic in vitro model of the colon (TIM-2). CFE was provided at two doses (250 and 350 mg/day) for 3 days. CFE led to a dose-dependent increase in Roseburia, Eubacterium ramulus, and Bacteroides eggerthii. There was a shift in production of short-chain fatty acids, where acetate production increased on CFE, while butyrate decreased. In overweight and obesity, acetate has been shown to increase fat oxidation when produced in the distal gut, and stimulate secretion of appetite-suppressive neuropeptides. Thus, the data in the in vitro model point towards mechanisms underlying the effects of the polyphenols in CFE with respect to modulation of the gut microbiota, both in composition and activity. These results should be confirmed in a clinical trial.

KW - Roseburia

KW - Citrus Fruit Extract

KW - gut microbiota

KW - short-chain fatty acids (SCFA)

KW - in vitro model

KW - TIM-2

KW - CHAIN FATTY-ACIDS

KW - METABOLIC SYNDROME

KW - FECAL MICROBIOTA

KW - LARGE-INTESTINE

KW - PROPIONIC-ACID

KW - FERMENTATION

KW - ACETATE

KW - IMPACT

KW - PHYSIOLOGY

KW - INFUSIONS

U2 - 10.3390/nu13113915

DO - 10.3390/nu13113915

M3 - Article

C2 - 34836169

SN - 2072-6643

VL - 13

JO - Nutrients

JF - Nutrients

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ER -