Exogenously Added Oxyphytosterols Do Not Affect Macrophage-Mediated Inflammatory Responses

Yvonne Oligschlaeger, Tom Houben, Mike L. J. Jeurissen, Albert V. Bitorina, Maurice Konings, Sabine Baumgartner, Jogchum Plat, Ronit Shiri-Sverdlov*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Although phytosterols, plant-derived sterol-like components, are well known for their cholesterol-lowering properties, their atherogenic potential is still under debate. Although they are known to share structural similarities with cholesterol, it is unclear whether their oxidized forms (oxyphytosterols) have the capacity to mediate proinflammatory responses in macrophages. In the present study, bone marrow-derived macrophages were treated with oxidized low-density lipoproteins, oxyphytosterols (7keto-sito/campesterol [7keto-sit/camp] or 7-beta-hydroxy-sito/campesterol [7OH-sit/camp]), nonoxidized phytosterol (-sitosterol), or carrier-control (cyclodextrin) in a dose- and time-dependent manner. Inflammatory cytokine release, activity, and the corresponding mRNA expression levels were analyzed. 7OH-sit/camp, rather than 7keto-sit/camp, induced a modest proinflammatory response in wild-type cells derived from C57Bl/6 mice. The observed mild inflammatory effects are independent of the low-density lipoprotein receptor and Cluster of differentiation 36/Scavenger receptor-a. These data suggest that exogenously added oxyphytosterols do not affect macrophage-mediated inflammatory responses, at least in vitro.

Original languageEnglish
Pages (from-to)457-462
Number of pages6
JournalLipids
Volume53
Issue number4
DOIs
Publication statusPublished - Apr 2018

Keywords

  • Bone marrow-derived macrophages
  • Inflammation
  • Oxyphytosterols
  • Sitosterol
  • PHYTOSTEROL OXIDATION-PRODUCTS
  • CORONARY-ARTERY-DISEASE
  • LOW-DENSITY-LIPOPROTEIN
  • SEVERE AORTIC-STENOSIS
  • OXIDIZED LDL
  • DIETARY PHYTOSTEROLS
  • ATHEROSCLEROSIS
  • MICE
  • STEROLS
  • PLASMA

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