3D-printed bioactive scaffolds from nanosilicates and PEOT/PBT for bone tissue engineering

James K. Carrow; Andrea Di Luca; Alireza Dolatshahi-Pirouz; Lorenzo Moroni; Akhilesh K. Gaharwar

doi:10.1093/rb/rby024

3D-printed bioactive scaffolds from nanosilicates and PEOT/PBT for bone tissue engineering

James K. Carrow, Andrea Di Luca, Alireza Dolatshahi-Pirouz, Lorenzo Moroni^*, Akhilesh K. Gaharwar^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Additive manufacturing (AM) has shown promise in designing 3D scaffold for regenerative medicine. However, many synthetic biomaterials used for AM are bioinert. Here, we report synthesis of bioactive nanocomposites from a poly(ethylene oxide terephthalate) (PEOT)/poly(butylene terephthalate) (PBT) (PEOT/PBT) copolymer and 2D nanosilicates for fabricating 3D scaffolds for bone tissue engineering. PEOT/PBT have been shown to support calcification and bone bonding ability in vivo, while 2D nanosilicates induce osteogenic differentiation of human mesenchymal stem cells (hMSCs) in absence of osteoinductive agents. The effect of nanosilicates addition to PEOT/PBT on structural, mechanical and biological properties is investigated. Specifically, the addition of nanosilicate to PEOT/PBT improves the stability of nanocomposites in physiological conditions, as nanosilicate suppressed the degradation rate of copolymer. However, no significant increase in the mechanical stiffness of scaffold due to the addition of nanosilicates is observed. The addition of nanosilicates to PEOT/PBT improves the bioactive properties of AM nanocomposites as demonstrated in vitro. hMSCs readily proliferated on the scaffolds containing nanosilicates and resulted in significant upregulation of osteo-related proteins and production of mineralized matrix. The synergistic ability of nanosilicates and PEOT/PBT can be utilized for designing bioactive scaffolds for bone tissue engineering.

Original language	English
Pages (from-to)	29-37
Number of pages	9
Journal	Regenerative biomaterials
Volume	6
Issue number	1
DOIs	https://doi.org/10.1093/rb/rby024
Publication status	Published - Feb 2019

Keywords

3D printing
nanocomposites
two dimensional (2D) nanoparticles
copolymer
tissue engineering
OSTEOGENIC DIFFERENTIATION
IN-VITRO
NANOENGINEERED HYDROGELS
POLY(ETHYLENE GLYCOL)
MECHANICAL-PROPERTIES
COPOLYMER SCAFFOLDS
CARTILAGE REPAIR
POLYACTIVE(R)
DESIGN
NANOMATERIALS

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Cite this

@article{ce7ceab9827e4b55a589998f3c796f49,

title = "3D-printed bioactive scaffolds from nanosilicates and PEOT/PBT for bone tissue engineering",

abstract = "Additive manufacturing (AM) has shown promise in designing 3D scaffold for regenerative medicine. However, many synthetic biomaterials used for AM are bioinert. Here, we report synthesis of bioactive nanocomposites from a poly(ethylene oxide terephthalate) (PEOT)/poly(butylene terephthalate) (PBT) (PEOT/PBT) copolymer and 2D nanosilicates for fabricating 3D scaffolds for bone tissue engineering. PEOT/PBT have been shown to support calcification and bone bonding ability in vivo, while 2D nanosilicates induce osteogenic differentiation of human mesenchymal stem cells (hMSCs) in absence of osteoinductive agents. The effect of nanosilicates addition to PEOT/PBT on structural, mechanical and biological properties is investigated. Specifically, the addition of nanosilicate to PEOT/PBT improves the stability of nanocomposites in physiological conditions, as nanosilicate suppressed the degradation rate of copolymer. However, no significant increase in the mechanical stiffness of scaffold due to the addition of nanosilicates is observed. The addition of nanosilicates to PEOT/PBT improves the bioactive properties of AM nanocomposites as demonstrated in vitro. hMSCs readily proliferated on the scaffolds containing nanosilicates and resulted in significant upregulation of osteo-related proteins and production of mineralized matrix. The synergistic ability of nanosilicates and PEOT/PBT can be utilized for designing bioactive scaffolds for bone tissue engineering.",

keywords = "3D printing, nanocomposites, two dimensional (2D) nanoparticles, copolymer, tissue engineering, OSTEOGENIC DIFFERENTIATION, IN-VITRO, NANOENGINEERED HYDROGELS, POLY(ETHYLENE GLYCOL), MECHANICAL-PROPERTIES, COPOLYMER SCAFFOLDS, CARTILAGE REPAIR, POLYACTIVE(R), DESIGN, NANOMATERIALS",

author = "Carrow, {James K.} and {Di Luca}, Andrea and Alireza Dolatshahi-Pirouz and Lorenzo Moroni and Gaharwar, {Akhilesh K.}",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2018. Published by Oxford University Press.",

year = "2019",

month = feb,

doi = "10.1093/rb/rby024",

language = "English",

volume = "6",

pages = "29--37",

journal = "Regenerative biomaterials",

issn = "2056-3418",

publisher = "Oxford University Press",

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TY - JOUR

T1 - 3D-printed bioactive scaffolds from nanosilicates and PEOT/PBT for bone tissue engineering

AU - Carrow, James K.

AU - Di Luca, Andrea

AU - Dolatshahi-Pirouz, Alireza

AU - Moroni, Lorenzo

AU - Gaharwar, Akhilesh K.

N1 - Publisher Copyright: © The Author(s) 2018. Published by Oxford University Press.

PY - 2019/2

Y1 - 2019/2

N2 - Additive manufacturing (AM) has shown promise in designing 3D scaffold for regenerative medicine. However, many synthetic biomaterials used for AM are bioinert. Here, we report synthesis of bioactive nanocomposites from a poly(ethylene oxide terephthalate) (PEOT)/poly(butylene terephthalate) (PBT) (PEOT/PBT) copolymer and 2D nanosilicates for fabricating 3D scaffolds for bone tissue engineering. PEOT/PBT have been shown to support calcification and bone bonding ability in vivo, while 2D nanosilicates induce osteogenic differentiation of human mesenchymal stem cells (hMSCs) in absence of osteoinductive agents. The effect of nanosilicates addition to PEOT/PBT on structural, mechanical and biological properties is investigated. Specifically, the addition of nanosilicate to PEOT/PBT improves the stability of nanocomposites in physiological conditions, as nanosilicate suppressed the degradation rate of copolymer. However, no significant increase in the mechanical stiffness of scaffold due to the addition of nanosilicates is observed. The addition of nanosilicates to PEOT/PBT improves the bioactive properties of AM nanocomposites as demonstrated in vitro. hMSCs readily proliferated on the scaffolds containing nanosilicates and resulted in significant upregulation of osteo-related proteins and production of mineralized matrix. The synergistic ability of nanosilicates and PEOT/PBT can be utilized for designing bioactive scaffolds for bone tissue engineering.

AB - Additive manufacturing (AM) has shown promise in designing 3D scaffold for regenerative medicine. However, many synthetic biomaterials used for AM are bioinert. Here, we report synthesis of bioactive nanocomposites from a poly(ethylene oxide terephthalate) (PEOT)/poly(butylene terephthalate) (PBT) (PEOT/PBT) copolymer and 2D nanosilicates for fabricating 3D scaffolds for bone tissue engineering. PEOT/PBT have been shown to support calcification and bone bonding ability in vivo, while 2D nanosilicates induce osteogenic differentiation of human mesenchymal stem cells (hMSCs) in absence of osteoinductive agents. The effect of nanosilicates addition to PEOT/PBT on structural, mechanical and biological properties is investigated. Specifically, the addition of nanosilicate to PEOT/PBT improves the stability of nanocomposites in physiological conditions, as nanosilicate suppressed the degradation rate of copolymer. However, no significant increase in the mechanical stiffness of scaffold due to the addition of nanosilicates is observed. The addition of nanosilicates to PEOT/PBT improves the bioactive properties of AM nanocomposites as demonstrated in vitro. hMSCs readily proliferated on the scaffolds containing nanosilicates and resulted in significant upregulation of osteo-related proteins and production of mineralized matrix. The synergistic ability of nanosilicates and PEOT/PBT can be utilized for designing bioactive scaffolds for bone tissue engineering.

KW - 3D printing

KW - nanocomposites

KW - two dimensional (2D) nanoparticles

KW - copolymer

KW - tissue engineering

KW - OSTEOGENIC DIFFERENTIATION

KW - IN-VITRO

KW - NANOENGINEERED HYDROGELS

KW - POLY(ETHYLENE GLYCOL)

KW - MECHANICAL-PROPERTIES

KW - COPOLYMER SCAFFOLDS

KW - CARTILAGE REPAIR

KW - POLYACTIVE(R)

KW - DESIGN

KW - NANOMATERIALS

U2 - 10.1093/rb/rby024

DO - 10.1093/rb/rby024

M3 - Article

C2 - 30740240

SN - 2056-3418

VL - 6

SP - 29

EP - 37

JO - Regenerative biomaterials

JF - Regenerative biomaterials

IS - 1

ER -