3D fiber deposited polymeric scaffolds for external auditory canal wall

Carlos Mota, Mario Milazzo, Daniele Panetta, Luisa Trombi, Vera Gramigna, Piero A. Salvadori, Stefano Giannotti, Luca Bruschini, Cesare Stefanini, Lorenzo Moroni, Stefano Berrettini, Serena Danti*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

The external auditory canal (EAC) is an osseocartilaginous structure extending from the auricle to the eardrum, which can be affected by congenital, inflammatory, and neoplastic diseases, thus reconstructive materials are needed. Current biomaterialbased approaches for the surgical reconstruction of EAC posterior wall still suffer from resorption (biological) and extrusion (synthetic). In this study, 3D fiber deposited scaffolds based on poly(ethylene oxide terephthalate)/ poly(butylene terephthalate) were designed and fabricated to replace the EAC wall. Fiber diameter and scaffold porosity were optimized, leading to 200 +/- 33 mu m and 55% +/- 5%, respectively. The mechanical properties were evaluated, resulting in a Young's modulus of 25.1 +/- 7.0 MPa. Finally, the EAC scaffolds were tested in vitro with osteo-differentiated human mesenchymal stromal cells (hMSCs) with different seeding methods to produce homogeneously colonized replacements of interest for otologic surgery. This study demonstrated the fabrication feasibility of EAC wall scaffolds aimed to match several important requirements for biomaterial application to the ear under the Tissue Engineering paradigm, including shape, porosity, surface area, mechanical properties and favorable in vitro interaction with osteoinduced hMSCs.

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Original languageEnglish
Article number63
Number of pages14
JournalJournal of Materials Science-Materials in Medicine
Volume29
Issue number5
DOIs
Publication statusPublished - May 2018
Event28th Annual Conference of the European-Society-for-Biomaterials (ESB) - Athens, Greece
Duration: 4 Sep 20178 Sep 2017

Keywords

  • DYNAMIC-MECHANICAL PROPERTIES
  • TISSUE ENGINEERING APPLICATIONS
  • PEOT/PBT COPOLYMER SCAFFOLDS
  • IMAGE-ANALYSIS
  • BIOCOMPATIBILITY
  • ARCHITECTURE
  • CARTILAGE
  • GEOMETRY
  • POROSITY

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