Overcoming kidney organoid challenges for regenerative medicine

Thomas Geuens; Clemens A. van Blitterswijk; Vanessa L. S. LaPointe

doi:10.1038/s41536-020-0093-4

Overcoming kidney organoid challenges for regenerative medicine

Thomas Geuens, Clemens A. van Blitterswijk, Vanessa L. S. LaPointe^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Kidney organoids derived from human induced pluripotent stem cells bear the potential to be used as a regenerative medicine renal replacement therapy. Advances in developmental biology shed light on the complex cellular regulation during kidney morphogenesis in animal models resulting in insights that were incorporated in the development of groundbreaking protocols for the directed differentiation of human pluripotent stem cells to kidney endpoints. Moreover, further optimization efforts to improve three-dimensional culture techniques resulted in the creation of kidney organoids. Before they can find their way to the clinic, there are critical challenges to overcome. Here, we will discuss recent advances and remaining challenges for kidney organoids to become successful in regenerative medicine. An innovative combination of tissue engineering techniques with more refined insights in the developing human kidney will ultimately lead to more mature and functional kidney organoids suitable as renal replacement therapy for patients with chronic kidney disease.

Original language	English
Article number	8
Number of pages	6
Journal	Npj regenerative medicine
Volume	5
Issue number	1
DOIs	https://doi.org/10.1038/s41536-020-0093-4
Publication status	Published - 30 Apr 2020

Keywords

PLURIPOTENT STEM-CELLS
GENERATION
DIFFERENTIATION
CONSTRUCTS
CULTURE

Access to Document

10.1038/s41536-020-0093-4Licence: CC BY

Cite this

@article{32328519f06147ab8f0c3e01cd793a8d,

title = "Overcoming kidney organoid challenges for regenerative medicine",

abstract = "Kidney organoids derived from human induced pluripotent stem cells bear the potential to be used as a regenerative medicine renal replacement therapy. Advances in developmental biology shed light on the complex cellular regulation during kidney morphogenesis in animal models resulting in insights that were incorporated in the development of groundbreaking protocols for the directed differentiation of human pluripotent stem cells to kidney endpoints. Moreover, further optimization efforts to improve three-dimensional culture techniques resulted in the creation of kidney organoids. Before they can find their way to the clinic, there are critical challenges to overcome. Here, we will discuss recent advances and remaining challenges for kidney organoids to become successful in regenerative medicine. An innovative combination of tissue engineering techniques with more refined insights in the developing human kidney will ultimately lead to more mature and functional kidney organoids suitable as renal replacement therapy for patients with chronic kidney disease.",

keywords = "PLURIPOTENT STEM-CELLS, GENERATION, DIFFERENTIATION, CONSTRUCTS, CULTURE",

author = "Thomas Geuens and {van Blitterswijk}, {Clemens A.} and LaPointe, {Vanessa L. S.}",

note = "Funding Information: This work is supported by the partners of Regenerative Medicine Crossing Borders (RegMed XB), a public-private partnership that uses regenerative medicine strategies to cure common chronic diseases. This collaboration is financed by the Dutch Ministry of Economic Affairs by means of the PPP Allowance made available by the Top Sector Life Sciences & Health to stimulate public-private partnerships. The authors also thank the Dutch Province of Limburg. Publisher Copyright: {\textcopyright} 2020, The Author(s).",

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AU - van Blitterswijk, Clemens A.

AU - LaPointe, Vanessa L. S.

N1 - Funding Information: This work is supported by the partners of Regenerative Medicine Crossing Borders (RegMed XB), a public-private partnership that uses regenerative medicine strategies to cure common chronic diseases. This collaboration is financed by the Dutch Ministry of Economic Affairs by means of the PPP Allowance made available by the Top Sector Life Sciences & Health to stimulate public-private partnerships. The authors also thank the Dutch Province of Limburg. Publisher Copyright: © 2020, The Author(s).

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N2 - Kidney organoids derived from human induced pluripotent stem cells bear the potential to be used as a regenerative medicine renal replacement therapy. Advances in developmental biology shed light on the complex cellular regulation during kidney morphogenesis in animal models resulting in insights that were incorporated in the development of groundbreaking protocols for the directed differentiation of human pluripotent stem cells to kidney endpoints. Moreover, further optimization efforts to improve three-dimensional culture techniques resulted in the creation of kidney organoids. Before they can find their way to the clinic, there are critical challenges to overcome. Here, we will discuss recent advances and remaining challenges for kidney organoids to become successful in regenerative medicine. An innovative combination of tissue engineering techniques with more refined insights in the developing human kidney will ultimately lead to more mature and functional kidney organoids suitable as renal replacement therapy for patients with chronic kidney disease.

AB - Kidney organoids derived from human induced pluripotent stem cells bear the potential to be used as a regenerative medicine renal replacement therapy. Advances in developmental biology shed light on the complex cellular regulation during kidney morphogenesis in animal models resulting in insights that were incorporated in the development of groundbreaking protocols for the directed differentiation of human pluripotent stem cells to kidney endpoints. Moreover, further optimization efforts to improve three-dimensional culture techniques resulted in the creation of kidney organoids. Before they can find their way to the clinic, there are critical challenges to overcome. Here, we will discuss recent advances and remaining challenges for kidney organoids to become successful in regenerative medicine. An innovative combination of tissue engineering techniques with more refined insights in the developing human kidney will ultimately lead to more mature and functional kidney organoids suitable as renal replacement therapy for patients with chronic kidney disease.

KW - PLURIPOTENT STEM-CELLS

KW - GENERATION

KW - DIFFERENTIATION

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KW - CULTURE

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