TY - JOUR
T1 - Diet-induced weight loss reduces postprandial dicarbonyl stress in abdominally obese men
T2 - Secondary analysis of a randomized controlled trial
AU - Van den Eynde, Mathias D. G.
AU - Kusters, Yvo H. A. M.
AU - Houben, Alfons J. H. M.
AU - Scheijen, Jean L. J. M.
AU - van Duynhoven, John
AU - Fazelzadeh, Parastoo
AU - Joris, Peter J.
AU - Plat, Jogchum
AU - Mensink, Ronald P.
AU - Hanssen, Nordin M. J.
AU - Stehouwer, Coen D. A.
AU - Schalkwijk, Casper G.
PY - 2021/5
Y1 - 2021/5
N2 - Aims: Dicarbonyl compounds contribute to the formation of advanced glycation endproducts (AGEs) and the development of insulin resistance and vascular complications. Dicarbonyl stress may already be detrimental in obesity. We evaluated whether diet-induced weight loss can effectively reverse dicarbonyl stress in abdominally obese men.Materials and methods: Plasma samples were collected from lean (n = 25) and abdominally obese men (n = 52) in the fasting state, and during a mixed meal test (MMT). Abdominally obese men were randomized to 8 weeks of dietary weight loss or habitual diet, followed by a second MMT. The a-dicarbonyls methylglyoxal (MGO), glyoxal (GO) and 3-deoxyglucosone (3-DG) and AGEs were measured by UPLC-MS/ MS. Skin autofluorescence (SAF) was measured using the AGE reader. T-tests were used for the crosssectional analysis and ANCOVA to assess the treatment effect.Results: Postprandial glucose, MGO and 3-DG concentrations were higher in obese men as compared to lean men (p < 0.05 for all). Fasting dicarbonyls, AGEs, and SAF were not different between lean and obese men. After the weight loss intervention, fasting MGO levels tended to decrease by 25 nmol/L (95%-CI:-51-0.5; p = 0.054). Postprandial dicarbonyls were decreased after weight loss as compared to the control group: iAUC of MGO decreased by 57% (5280 nmol/L.min; 95%-CI: 33-10526; p = 0.049), of GO by 66% (11,329 nmol/L.min; 95%-CI: 495-22162; p = 0.041), and of 3-DG by 45% (20,175 nmol/L.min; 95%-CI: 5351-35000; p = 0.009). AGEs and SAF did not change significantly after weight loss.Conclusion: Abdominal obesity is characterized by increased postprandial dicarbonyl stress, which can be reduced by a weight loss intervention. (C) 2021 The Authors. Published by Elsevier Ltd.
AB - Aims: Dicarbonyl compounds contribute to the formation of advanced glycation endproducts (AGEs) and the development of insulin resistance and vascular complications. Dicarbonyl stress may already be detrimental in obesity. We evaluated whether diet-induced weight loss can effectively reverse dicarbonyl stress in abdominally obese men.Materials and methods: Plasma samples were collected from lean (n = 25) and abdominally obese men (n = 52) in the fasting state, and during a mixed meal test (MMT). Abdominally obese men were randomized to 8 weeks of dietary weight loss or habitual diet, followed by a second MMT. The a-dicarbonyls methylglyoxal (MGO), glyoxal (GO) and 3-deoxyglucosone (3-DG) and AGEs were measured by UPLC-MS/ MS. Skin autofluorescence (SAF) was measured using the AGE reader. T-tests were used for the crosssectional analysis and ANCOVA to assess the treatment effect.Results: Postprandial glucose, MGO and 3-DG concentrations were higher in obese men as compared to lean men (p < 0.05 for all). Fasting dicarbonyls, AGEs, and SAF were not different between lean and obese men. After the weight loss intervention, fasting MGO levels tended to decrease by 25 nmol/L (95%-CI:-51-0.5; p = 0.054). Postprandial dicarbonyls were decreased after weight loss as compared to the control group: iAUC of MGO decreased by 57% (5280 nmol/L.min; 95%-CI: 33-10526; p = 0.049), of GO by 66% (11,329 nmol/L.min; 95%-CI: 495-22162; p = 0.041), and of 3-DG by 45% (20,175 nmol/L.min; 95%-CI: 5351-35000; p = 0.009). AGEs and SAF did not change significantly after weight loss.Conclusion: Abdominal obesity is characterized by increased postprandial dicarbonyl stress, which can be reduced by a weight loss intervention. (C) 2021 The Authors. Published by Elsevier Ltd.
KW - Abdominal obesity
KW - Weight loss intervention
KW - Dicarbonyl stress
KW - Advanced glycation endproducts (AGEs)
KW - Skin autofluorescence (SAF)
KW - ADVANCED GLYCATION
KW - OXIDATIVE STRESS
KW - ADIPOSE-TISSUE
KW - GLYOXALASE 1
KW - METHYLGLYOXAL
KW - INDIVIDUALS
KW - PENTOSIDINE
KW - METABOLISM
U2 - 10.1016/j.clnu.2021.03.042
DO - 10.1016/j.clnu.2021.03.042
M3 - Article
C2 - 33933731
SN - 0261-5614
VL - 40
SP - 2654
EP - 2662
JO - Clinical Nutrition
JF - Clinical Nutrition
IS - 5
ER -