TY - JOUR
T1 - 2′-Fucosyllactose impacts the expression of mucus-related genes in goblet cells and maintains barrier function of gut epithelial cells
AU - Figueroa-Lozano, Susana
AU - Akkerman, Renate
AU - Beukema, Martin
AU - Leeuwen, S. Sander van
AU - Dijkhuizen, Lubbert
AU - de Vos, Paul
PY - 2021/10
Y1 - 2021/10
N2 - Scope: Cost-effective microbial biosynthesis of 2'-fucosyllactose (2'-FL) allows its application in infant formula. The specific effects of 2'-FL on the gastrointestinal immune barrier are still largely unknown.Methods/results: Here, we quantified and compared the effects of HMOs isolated from human milk, 2'-FL/lactose (Lac), and 2'-FL on the expression of the mucus associated genes MUC2, TFF3, RETLB and the Golgi sulfo-transferases, CHST5, and GAL3ST5, in human goblet cells. We also determined whether these compounds have protective effects on A23187-induced barrier disruption of human T84 gut epithelial cells in vitro. The impact of isolated HMOs and 2'-FL/Lac on the mRNA expression of the mucus-related genes was minor while pure 2'-FL significantly induced GAL3ST2 and CHST5. Isolated HMOs, 2'-FL/Lac and 2'-FL all prevented A23187-induced barrier disruption in human T84 cells.Conclusion: Our findings indicate that 2'-FL modulates the secretory function of goblet cells and protects gut epithelial cells.
AB - Scope: Cost-effective microbial biosynthesis of 2'-fucosyllactose (2'-FL) allows its application in infant formula. The specific effects of 2'-FL on the gastrointestinal immune barrier are still largely unknown.Methods/results: Here, we quantified and compared the effects of HMOs isolated from human milk, 2'-FL/lactose (Lac), and 2'-FL on the expression of the mucus associated genes MUC2, TFF3, RETLB and the Golgi sulfo-transferases, CHST5, and GAL3ST5, in human goblet cells. We also determined whether these compounds have protective effects on A23187-induced barrier disruption of human T84 gut epithelial cells in vitro. The impact of isolated HMOs and 2'-FL/Lac on the mRNA expression of the mucus-related genes was minor while pure 2'-FL significantly induced GAL3ST2 and CHST5. Isolated HMOs, 2'-FL/Lac and 2'-FL all prevented A23187-induced barrier disruption in human T84 cells.Conclusion: Our findings indicate that 2'-FL modulates the secretory function of goblet cells and protects gut epithelial cells.
KW - 2 '-fucosyllactose
KW - Barrier function
KW - Goblet cells
KW - Human milk oligosaccharides (HMOs)
KW - Teer
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=researchintelligenceproject&SrcAuth=WosAPI&KeyUT=WOS:000686899700003&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.1016/j.jff.2021.104630
DO - 10.1016/j.jff.2021.104630
M3 - Article
SN - 1756-4646
VL - 85
JO - Journal of Functional Foods
JF - Journal of Functional Foods
M1 - 104630
ER -