Abstract
Osteoarthritis (OA) is a degenerative joint disease characterized by irreversible cartilage damage, inflammation and altered chondrocyte phenotype. Transforming growth factor-beta (TGF-beta) signaling via SMAD2/3 is crucial for blocking hypertrophy. The post-translational modifications of these SMAD proteins in the linker domain regulate their function and these can be triggered by inflammation through the activation of kinases or phosphatases. Therefore, we investigated if OA-related inflammation affects TGF-beta signaling via SMAD2/3 linker-modifications in chondrocytes. We found that both Interleukin (IL)-1 beta and OA-synovium conditioned medium negated SMAD2/3 transcriptional activity in chondrocytes. This inhibition of TGF-beta signaling was enhanced if SMAD3 could not be phosphorylated on Ser213 in the linker region and the inhibition by IL-1 beta was less if the SMAD3 linker could not be phosphorylated at Ser204. Our study shows evidence that inflammation inhibits SMAD2/3 signaling in chondrocytes via SMAD linker (de)-phosphorylation. The involvement of linker region modifications may represent a new therapeutic target for OA.
Original language | English |
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Article number | 8124 |
Number of pages | 22 |
Journal | International Journal of Molecular Sciences |
Volume | 22 |
Issue number | 15 |
DOIs | |
Publication status | Published - 1 Aug 2021 |
Keywords
- TGF-beta
- osteoarthritis
- cartilage
- SMAD2
- 3 signaling
- linker modifications
- inflammation
- TERMINAL DOMAIN PHOSPHATASES
- ARTICULAR CHONDROCYTES
- TUMOR-SUPPRESSION
- GENE-EXPRESSION
- DOWN-REGULATION
- UP-REGULATION
- PHOSPHORYLATION
- CARTILAGE
- CYTOKINES
- INTERLEUKIN-1-BETA