@article{0f5c8fd6163c43f7b3348664ec35d000,
title = "Simultaneous Detection of Zinc and Its Pathway Metabolites Using MALDI MS Imaging of Prostate Tissue",
abstract = "Levels of zinc, along with its mechanistically related metabolites citrate and aspartate, are widely reported as reduced in prostate cancer compared to healthy tissue and are therefore pointed out as potential cancer biomarkers. Previously, it has only been possible to analyze zinc and metabolites by separate detection methods. Through matrix-assisted laser desorption/ionization mass spectrometry imaging (MSI), we were for the first time able to demonstrate, in two different sample sets (n = 45 and n = 4), the simultaneous spatial detection of zinc, in the form of ZnCl3-, together with citrate, aspartate, and N-acetylaspartate on human prostate cancer tissues. The reliability of the ZnCl3- detection was validated by total zinc determination using laser ablation inductively coupled plasma MSI on adjacent serial tissue sections. Zinc, citrate, and aspartate were correlated with each other (range r = 0.46 to 0.74) and showed a significant reduction in cancer compared to non-cancer epithelium (p <0.05, log(2) fold change range: -0.423 to -0.987), while no significant difference between cancer and stroma tissue was found. Simultaneous spatial detection of zinc and its metabolites is not only a valuable tool for analyzing the role of zinc in prostate metabolism but might also provide a fast and simple method to detect zinc, citrate, and aspartate levels as a biomarker signature for prostate cancer diagnostics and prognostics.",
keywords = "MASS-SPECTROMETRY, CANCER",
author = "Andersen, {Maria K.} and Sebastian Krossa and Hoiem, {Therese S.} and Rebecca Buchholz and Claes, {Britt S. R.} and Benjamin Balluff and Ellis, {Shane R.} and Elin Richardsen and Helena Bertilsson and Heeren, {Ron M. A.} and Bathen, {Tone F.} and Uwe Karst and Giskeodegard, {Guro F.} and May-Britt Tessem",
note = "Funding Information: This research was funded by the European Research Council (ERC) under the European Union{\textquoteright}s Horizon 2020 research and innovation program (grant agreement No. 758306), Norwegian University of Science and Technology (NTNU), Liaison Committee RHA-NTNU, Maastricht University, the LINK program of the Dutch province of Limburg, Norwegian Cancer Society, The Northern Health Administration, and UiT - The Arctic University of Norway. We thank Biobank1 (St. Olavs Hospital) for collecting and storing the core sample set. Sectioning of samples was performed by the cellular and molecular imaging core facility (CMIC) at NTNU Funding Information: This research was funded by the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement No. 758306), Norwegian University of Science and Technology (NTNU), Liaison Committee RHA-NTNU, Maastricht University, the LINK program of the Dutch province of Limburg, Norwegian Cancer Society, The Northern Health Administration, and UiT-The Arctic University of Norway. We thank Biobank1 (St. Olavs Hospital) for collecting and storing the core sample set. Sectioning of samples was performed by the cellular and molecular imaging core facility (CMIC) at NTNU. Publisher Copyright: Copyright {\textcopyright} 2020 American Chemical Society.",
year = "2020",
month = feb,
day = "18",
doi = "10.1021/acs.analchem.9b04903",
language = "English",
volume = "92",
pages = "3171--3179",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "4",
}