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Research Output

Acceleration of the direct identification of Staphylococcus aureus versus coagulase-negative staphylococci from blood culture material: a comparison of six bacterial DNA extraction methods

Loonen, A. J. M., Jansz, A. R., Kreeftenberg, H., Bruggeman, C. A., Wolffs, P. F. G. & van den Brule, A. J. C., Mar 2011, In : European Journal of Clinical Microbiology & Infectious Diseases. 30, 3, p. 337-342

Research output: Contribution to journalArticleAcademicpeer-review

Anorectal Chlamydia trachomatis Load Is Similar in Men Who Have Sex with Men and Women Reporting Anal Sex

van Liere, G. A. F. S., Dirks, J. A. M. C., Hoebe, C. J. P. A., Wolffs, P. F. & Dukers-Muijrers, N. H. T. M., 11 Aug 2015, In : PLOS ONE. 10, 8, 12 p., e0134991.

Research output: Contribution to journalArticleAcademicpeer-review

Antibiotic Susceptibility Testing of Grown Blood Cultures by Combining Culture and Real-Time Polymerase Chain Reaction Is Rapid and Effective

Beuving, J., Verbon, A., Gronthoud, F. A., Stobberingh, E. E. & Wolffs, P. F. G., 14 Dec 2011, In : PLOS ONE. 6, 12, e27689.

Research output: Contribution to journalArticleAcademicpeer-review

Antibiotic Use Before Chlamydia and Gonorrhea Genital and Extragenital Screening in the Sexually Transmitted Infection Clinical Setting

Dukers-Muijrers, N. H. T. M., van Liere, G. A. F. S., Wolffs, P. F. G., den Heijer, C., Werner, M. I. L. S. & Hoebe, C. J. P. A., 1 Jan 2015, In : Antimicrobial Agents and Chemotherapy. 59, 1, p. 121-128 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

A Real-Time PCR-Based Semi-Quantitative Breakpoint to Aid in Molecular Identification of Urinary Tract Infections.

Hansen, W. L. J., van der Donk, C. F., Bruggeman, C. A., Stobberingh, E. E. & Wolffs, P. F., 1 Jan 2013, In : PLOS ONE. 8, 4, e61439.

Research output: Contribution to journalArticleAcademicpeer-review

Assessment of herd effects among women and heterosexual men after girls-only HPV16/18 vaccination in the Netherlands: A repeated cross-sectional study

Woestenberg, P. J., Bogaards, J. A., King, A. J., Leussink, S., van der Sande, M. A. B., Hoebe, C. J. P. A., van Benthem, B. H. B., Adema, D., Buist-Arkema, R., Luijt, D., Meijer, S., Schirm, J., Buiting, T. A., Verbakel, H., van Esch, P., Verweij, J., van der Eijk, A., Lunel, F. V., Lakbiach, S., Schuurman, R. & 31 others, Abma, D., Adams, K., Bruisten, S., Linde, P., Oostvogel, P., Touwen, C., Vermeulen, W., Nelissen, J., Wolffs, P., van Duijvendijk, N., Schneeberger, P., Dinnissen-van Poppel, M., Melchers, W., Hooghiemstra, M., Huisman, H., Weel, J., Bosma, F., Geeraedts, F., Polman, van Goor, P., Wolthagen, M., de Mooij, C., van Koolwijk, E., Peters, M., Swanink, C., Janssen, J., Pelsers, M., Niekamp, A. M., Smit, M., van Rooijen, M. & Med Microbiological Labs Public Hl, 1 Jun 2019, In : International Journal of Cancer. 144, 11, p. 2718-2727 10 p.

Research output: Contribution to journalArticleAcademicpeer-review

Open Access

Assessment of rectal Chlamydia trachomatis viable load in women by viability-PCR

Janssen, K. J. H., Wolffs, P., Lucchesi, M., Dukers-Muijrers, N. H. T. M. & Hoebe, C. J. P. A., Mar 2020, In : Sexually Transmitted Infections. 96, 2, p. 85-88 4 p.

Research output: Contribution to journalArticleAcademicpeer-review

Bivalent Vaccine Effectiveness Against Type-Specific HPV Positivity: Evidence for Cross-Protection Against Oncogenic Types Among Dutch STI Clinic Visitors

Woestenberg, P. J., King, A. J., van Benthem, B. H. B., Donken, R., Leussink, S., van der Klis, F. R. M., de Melker, H. E., van der Sande, M. A. B., Hoebe, C. J. P. A., Med Microbiological Labs Public, Wolffs, P. & Bogaards, J. A., 15 Jan 2018, In : Journal of Infectious Diseases. 217, 2, p. 213-222 10 p.

Research output: Contribution to journalArticleAcademicpeer-review

Open Access
Open Access

Chlamydia trachomatis bacterial load, estimated by Cq values, in urogenital samples from men and women visiting the general practice, hospital or STI clinic

Wijers, J. N. A. P., Hoebe, C. J. P. A., van Liere, G. A. F. S., Wolffs, P. F. G. & Dukers-Muijrers, N. H. T. M., 19 Apr 2019, In : PLOS ONE. 14, 4, 14 p., 0215606.

Research output: Contribution to journalArticleAcademicpeer-review

Open Access

Chlamydia trachomatis Load in Population-Based Screening and STI-Clinics: Implications for Screening Policy

Dirks, J. A. M. C., Wolffs, P. F. G., Dukers-Muijrers, N. H. T. M., Brink, A. A. T. P., Speksnijders, A. G. C. L. & Hoebe, C. J. P. A., 1 Jan 2015, In : PLOS ONE. 10, 3, e0121433.

Research output: Contribution to journalArticleAcademicpeer-review

CMV DNA levels and CMV gB subtypes in ART-naive HAART-treated patients: a 2-year follow-up study in The Netherlands

Goossens, V. J., Wolffs, P. F., van Loo, I. H., Bruggeman, C. A. & Verbon, A., 1 Jan 2009, In : Aids. 23, 11, p. 1425-9

Research output: Contribution to journalArticleAcademicpeer-review

Comparison of three different techniques for the isolation of viral RNA in sputum

Vanspauwen, M. J., Wolffs, P. F. G., Franssen, F. M. E., Bruggeman, C. A., Wouters, E. F. M. & Linssen, C. F. M., Oct 2014, In : Journal of Clinical Virology. 61, 2, p. 265-269 5 p.

Research output: Contribution to journalArticleAcademicpeer-review

Comparison of urine samples and penile swabs for detection of human papillomavirus in HIV-negative Dutch men

Koene, F., Wolffs, P., Brink, A., Dukers-Muijrers, N., Quint, W., Bruggeman, C. & Hoebe, C., Sep 2016, In : Sexually Transmitted Infections. 92, 6, p. 467-469 3 p.

Research output: Contribution to journalArticleAcademicpeer-review

Compartmentalization of Acyclovir-Resistant Varicella Zoster Virus: Implications for Sampling in Molecular Diagnostics

Brink, A. A. T. P., van Gelder, M., Wolffs, P. F., Bruggeman, C. A. & van Loo, I. H. M., 15 Apr 2011, In : Clinical Infectious Diseases. 52, 8, p. 982-987

Research output: Contribution to journalArticleAcademicpeer-review

Correlation between viral load, multiplicity of infection, and persistence of HPV16 and HPV18 infection in a Dutch cohort of young women

van der Weele, P., van Logchem, E., Wolffs, P., van den Broek, I., Feltkamp, M., de Melker, H., Meijer, C. J. L. M., Boot, H. & King, A. J., Oct 2016, In : Journal of Clinical Virology. 83, p. 6-11 6 p.

Research output: Contribution to journalArticleAcademicpeer-review

Design of the FemCure study: prospective multicentre study on the transmission of genital and extra-genital Chlamydia trachomatis infections in women receiving routine care

Dukers-Muijrers, N. H. T. M., Wolffs, P. F. G., Eppings, L., Götz, H. M., Bruisten, S. M., Schim van der Loeff, M. F., Janssen, K., Lucchesi, M., Heijman, T., van Benthem, B. H., van Bergen, J. E., Morre, S. A., Herbergs, J., Kok, G., Steenbakkers, M., Hogewoning, A. A., de Vries, H. J. & Hoebe, C. J. P. A., 8 Aug 2016, In : BMC Infectious Diseases. 16, 9 p., 381.

Research output: Contribution to journalArticleAcademicpeer-review

Open Access
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Detection of Anorectal and Cervicovaginal Chlamydia Trachomatis Infections following Azithromycin Treatment: Prospective Cohort Study with Multiple Time-Sequential Measures of rRNA, DNA, Quantitative Load and Symptoms

Dukers-Muijrers, N. H. T. M., Speksnijder, A. G. C. L., Morre, S. A., Wolffs, P. F. G., van der Sande, M. A. B., Brink, A. A. T. P., van den Broek, I. V. F., Werner, M. I. L. S. & Hoebe, C. J. P. A., 20 Nov 2013, In : PLOS ONE. 8, 11, e81236.

Research output: Contribution to journalArticleAcademicpeer-review

Development and Validation of a Single-Tube Multiple-Locus Variable Number Tandem Repeat Analysis for Klebsiella pneumoniae

Brink, A. A. T. P., von Wintersdorff, C. J. H., van der Donk, C. F. M., Peeters, A. M. M. W., Beisser, P. S., Stobberingh, E. E. & Wolffs, P. F. G., 1 Jan 2014, In : PLOS ONE. 9, 3, e91209.

Research output: Contribution to journalArticleAcademicpeer-review

Developments for improved diagnosis of bacterial bloodstream infections

Loonen, A. J. M., Wolffs, P. F. G., Bruggeman, C. A. & van den Brule, A. J. C., 1 Jan 2014, In : European Journal of Clinical Microbiology & Infectious Diseases. 33, 10, p. 1687-1702

Research output: Contribution to journalArticleAcademicpeer-review

Differences in Virus Prevalence and Load in the Hearts of Patients with Idiopathic Dilated Cardiomyopathy with and without Immune-Mediated Inflammatory Diseases

Dennert, R., van Paassen, P., Wolffs, P., Bruggeman, C., Velthuis, S., Felix, S., van Suylen, R-J., Crijns, H. J., Tervaert, J. W. C. & Heymans, S., Aug 2012, In : Clinical and Vaccine Immunology. 19, 8, p. 1182-1187

Research output: Contribution to journalArticleAcademicpeer-review

Dissemination of Antimicrobial Resistance in Microbial Ecosystems through Horizontal Gene Transfer

von Wintersdorff, C., Penders, J., van Niekerk, J., Mills, N. D., Majumder, S., van Alphen, L., Savelkoul, P. H. M. & Wolffs, P. F. G., 19 Feb 2016, In : Frontiers in microbiology. 7, 10 p., 173.

Research output: Contribution to journalArticleAcademicpeer-review

Open Access

Enzymatic Digestion of Porcine Corneas Cross-linked by Hypo- and Hyperosmolar Formulations of Riboflavin/ultraviolet A or WST11/Near-Infrared Light

Brekelmans, J., Veugen, J., Rieff, K., Dickman, M. M., Goz, A., Wolffs, P., Brandis, A., Berendschot, T. T. J. M., Nuijts, R. M. M. A., Scherz, A. & Marcovich, A. L., Sep 2020, In : Translational Vision Science & Technology. 9, 10, 9 p., 4.

Research output: Contribution to journalArticleAcademicpeer-review

Open Access

Evaluation of direct inoculation of the BD PHOENIX system from positive BACTEC blood cultures for both Gram-positive cocci and Gram-negative rods

Beuving, J., van der Donk, C. F. M., Linssen, C. F. M., Wolffs, P. F. G. & Verbon, A., 30 Jun 2011, In : BMC Microbiology. 11, p. 7

Research output: Contribution to journalArticleAcademicpeer-review

Evaluation of phenotypic and molecular methods for identification of Streptococcus pneumoniae

Yahiaoui, R. Y., den Heijer, C. D. J., Wolffs, P., Bruggeman, C. & Stobberingh, E. E., 2016, In : Future Microbiology. 11, 1, p. 43-50 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

Genital and anal Chlamydia trachomatis bacterial load in concurrently infected women: a cross-sectional study

Dirks, J. A. M. C., van Liere, G. A. F. S., Hoebe, C. J. P. A., Wolffs, P. & Dukers-Muijrers, N. H. T. M., Aug 2019, In : Sexually Transmitted Infections. 95, 5, p. 317-321 5 p.

Research output: Contribution to journalArticleAcademicpeer-review

How to Count Our Microbes? The Effect of Different Quantitative Microbiome Profiling Approaches

Galazzo, G., van Best, N., Benedikter, B. J., Janssen, K., Bervoets, L., Driessen, C., Oomen, M., Lucchesi, M., van Eijck, P. H., Becker, H. E. F., Hornef, M. W., Savelkoul, P. H., Stassen, F. R. M., Wolffs, P. F. & Penders, J., 7 Aug 2020, In : Frontiers in Cellular and Infection Microbiology. 10, 10 p., 403.

Research output: Contribution to journalArticleAcademicpeer-review

Open Access

Impact of same-day antibiotic susceptibility testing on time to appropriate antibiotic treatment of patients with bacteraemia: a randomised controlled trial

Beuving, J., Wolffs, P. F. G., Hansen, W. L. J., Stobberingh, E. E., Bruggeman, C. A., Kessels, A. & Verbon, A., 1 Jan 2015, In : European Journal of Clinical Microbiology & Infectious Diseases. 34, 4, p. 831-838

Research output: Contribution to journalArticleAcademicpeer-review

Incidence and persistence of carcinogenic genital human papillomavirus infections in young women with or without Chlamydia trachomatis co-infection

Vriend, H. J., Bogaards, J. A., van Bergen, J. E. A. M., Brink, A. A. T. P., van den Broek, I. V. F., Hoebe, C. J. P. A., King, A. J., van der Sande, M. A. B., Wolffs, P. F. G., de Melker, H. E., Medical Microbiological Laboratories, T. & CSI group, T., 21 Jul 2015, In : Cancer Medicine. 4, 10, p. 1589-1598

Research output: Contribution to journalArticleAcademicpeer-review

Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR

van Dommelen, L., Wolffs, P. F. G., van Tiel, F., Dukers, N. H. T. M., Herngreen, S., Bruggeman, C. A. M. V. A. & Hoebe, C. J. P. A., Mar 2013, In : Journal of Clinical Microbiology. 51, 3, p. 990-992 3 p.

Research output: Contribution to journalArticleAcademicpeer-review

Interpretation of Real-time PCR Diagnosing Fecal Viruses in Children: A Prospective Case-control Study

Corcoran, M. S., van Loo, I. H., Wolffs, P. F. G. & van Well, G. T., Dec 2013, In : Pediatric Infectious Disease Journal. 32, 12, p. 1402-1403 2 p.

Research output: Contribution to journalComment/Letter to the editorAcademicpeer-review

Intravenous immunoglobulin therapy for patients with idiopathic cardiomyopathy and endomyocardial biopsy-proven high PVB19 viral load

Dennert, R., Velthuis, S., Schalla, S., Eurlings, L. W., van Suylen, R-J., van Paassen, P., Tervaert, J. W. C., Wolffs, P., Goossens, V. J., Bruggeman, C., Waltenberger, J., Crijns, H. J. & Heymans, S., 2010, In : Antiviral Therapy. 15, 2, p. 193-201

Research output: Contribution to journalArticleAcademicpeer-review

Lack of evidence for the role of human adenovirus-36 in obesity in a European cohort

Goossens, V. J., Dejager, S. A., Grauls, G. E., Gielen, M., Vlietinck, R. F., Derom, C. A., Loos, R. J., Rensen, S. S., Buurman, W. A., Greve, J. W., van Baak, M. A., Wolffs, P. F., Bruggeman, C. A. & Hoebe, C. J., 1 Jan 2011, In : Obesity. 19, 1, p. 220-221

Research output: Contribution to journalArticleAcademicpeer-review

Limits of Chlamydial Diagnostic Tests in Epidemiological Studies

Dukers-Muijrers, N. H. T. M., van der Sande, M. A. B., Wolffs, P. F. G. & Hoebe, C. J. P. A., 15 Jun 2014, In : Journal of Infectious Diseases. 209, 12, p. 2017-2018 2 p.

Research output: Contribution to journalComment/Letter to the editorAcademicpeer-review

Molecular Probes for Diagnosis of Clinically Relevant Bacterial Infections in Blood Cultures

Hansen, W. L. J., Beuving, J., Bruggeman, C. A. & Wolffs, P. F. G., Dec 2010, In : Journal of Clinical Microbiology. 48, 12, p. 4432-4438

Research output: Contribution to journalArticleAcademicpeer-review

Natural Course of Chlamydia trachomatis Bacterial Load in the Time Interval between Screening and Treatment in Anogenital Samples

Dirks, J. A. M. C., van Liere, G. A. F. S., Bogers, S., Dukers-Muijrers, N. H. T. M., Wolffs, P. F. G. & Hoebe, C. J. P. A., 1 Jan 2015, In : PLOS ONE. 10, 12, e0145693.

Research output: Contribution to journalArticleAcademicpeer-review

Oncogenic Viruses in Skull Base Chordomas

Yakkioui, Y., Speel, E-J. M., Van Overbeeke, J. J., Boderie, M. J. M., Pujari, S., Hausen, A. Z., Wolffs, P. F. G. & Temel, Y., 1 Apr 2018, In : World Neurosurgery. 112, p. E7-E13 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

One-day workflow scheme for bacterial pathogen detection and antimicrobial resistance testing from blood cultures

Hansen, W. L. J., Beuving, J., Verbon, A. & Wolffs, P. F. G., Jul 2012, In : Journal of visualized experiment. 65, 7 p., 3254.

Research output: Contribution to journalArticleAcademicpeer-review

Paediatric sepsis-like illness and human parechovirus

Vanagt, W. Y., Lutgens, S. P. M., van Loo, I. H. M. & Wolffs, P. F. G., May 2012, In : Archives of Disease in Childhood. 97, 5, p. 482-483 2 p.

Research output: Contribution to journalComment/Letter to the editorAcademicpeer-review

Participation, retention, and associated factors of women in a prospective multicenter study on Chlamydia trachomatis infections (FemCure)

Dukers-Muijrers, N. H. T. M., Heijman, T., Gotz, H. M., Zaandam, P., Wijers, J., Leenen, J., van Liere, G., Heil, J., Brinkhues, S., Wielemaker, A., van der Loeff, M. F. S., Wolffs, P. F. G., Bruisten, S. M., Steenbakkers, M., Hogewoning, A. A., de Vries, H. J. & Hoebe, C. J. P. A., 18 Mar 2020, In : PLOS ONE. 15, 3, 17 p., 0230413.

Research output: Contribution to journalArticleAcademicpeer-review

Open Access

Patterns of Human Papillomavirus DNA and Antibody Positivity in Young Males and Females, Suggesting a Site-Specific Natural Course of Infection

on behalf of the Medical Microbiological Laboratories, the Municipal Health Services & Hoebe, C., 23 Apr 2013, In : PLOS ONE. 8, 4, e60696.

Research output: Contribution to journalArticleAcademicpeer-review